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Pathogens 2015, 4(2), 210-228; doi:10.3390/pathogens4020210

Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)

1
Centro de Investigación en Dinámica Celular, Instituto de Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, CP 62209, Cuernavaca, Morelos, Mexico
2
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, 62209, Cuernavaca, Morelos, Mexico
3
El Colegio de la Frontera Sur, Carretera Panamericana y Periférico Sur s/n Barrio María Auxiliadora, CP 29290, San Cristóbal de Las Casas, Chiapas, Mexico
4
Facultad de Química, Universidad Nacional Autónoma de Mexico, Mexico D.F., Mexico
*
Author to whom correspondence should be addressed.
Academic Editor: Lawrence Young
Received: 20 November 2014 / Revised: 23 March 2015 / Accepted: 8 May 2015 / Published: 13 May 2015
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Abstract

Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology. View Full-Text
Keywords: Serratia marcescens; white grub; pathogenicity; Phyllophaga; scarab; biological control; insecticidal protein; serralysin Serratia marcescens; white grub; pathogenicity; Phyllophaga; scarab; biological control; insecticidal protein; serralysin
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pineda-Castellanos, M.L.; Rodríguez-Segura, Z.; Villalobos, F.J.; Hernández, L.; Lina, L.; Nuñez-Valdez, M.E. Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera). Pathogens 2015, 4, 210-228.

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