Next Article in Journal
Characterization of Antimicrobial Resistance Dissemination across Plasmid Communities Classified by Network Analysis
Next Article in Special Issue
Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
Previous Article in Journal
Pseudomonas aeruginosa Genome Evolution in Patients and under the Hospital Environment
Article Menu

Export Article

Open AccessArticle
Pathogens 2014, 3(2), 341-355; doi:10.3390/pathogens3020341

Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal Gold

Operational Technologies Corporation, 4100 N.W. Loop 410, Suite 230, San Antonio, TX 78229, USA
Received: 17 February 2014 / Revised: 30 March 2014 / Accepted: 1 April 2014 / Published: 10 April 2014
(This article belongs to the Special Issue Foodborne Pathogens)
View Full-Text   |   Download PDF [909 KB, uploaded 10 April 2014]   |  

Abstract

Preliminary studies aimed at improving the sensitivity of foodborne pathogen detection via lateral flow (LF) test strips by use of high affinity DNA aptamers for capture and reporter functions when coupled to red-emitting quantum dots (Qdot 655) are reported. A variety of DNA aptamers developed against Escherichia coli, Listeria monocytogenes, and Salmonella enterica were paired in capture and reporter combinations to determine which yielded the strongest detection of their cognate bacteria using a colloidal gold screening system. Several promising sandwich combinations were identified for each of the three bacterial LF strip systems. The best E. coli aptamer-LF system was further studied and yielded a visible limit of detection (LOD) of ~3,000 E. coli 8739 and ~6,000 E. coli O157:H7 in buffer. These LODs were reduced to ~300–600 bacterial cells per test respectively by switching to a Qdot 655 aptamer-LF system. Novel aspects of these assays such as the use of high levels of detergents to avoid quantum dot agglutination and enhance migration in analytical membranes, identification of optimal analytical membrane types, UV-immobilization of capture aptamers, and novel dual biotin/digoxigenin-end labeled aptamer streptavidin-colloidal gold or -Qdot 655 conjugates plus anti-digoxigenin antibody control lines are also discussed. In general, this work provides proof-of-principle for highly sensitive aptamer-Qdot LF strip assays for rapid foodborne pathogen detection. View Full-Text
Keywords: aptamer; colloidal gold; detergent; digoxigenin; E. coli ; lateral flow test strip; Listeria; quantum dot; Salmonella; SELEX aptamer; colloidal gold; detergent; digoxigenin; E. coli ; lateral flow test strip; Listeria; quantum dot; Salmonella; SELEX
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bruno, J.G. Application of DNA Aptamers and Quantum Dots to Lateral Flow Test Strips for Detection of Foodborne Pathogens with Improved Sensitivity versus Colloidal Gold. Pathogens 2014, 3, 341-355.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Pathogens EISSN 2076-0817 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top