Next Article in Journal
Honey Bee Survival and Pathogen Prevalence: From the Perspective of Landscape and Exposure to Pesticides
Next Article in Special Issue
Influence of Elytral Color Pattern, Size, and Sex of Harmonia axyridis (Coleoptera, Coccinellidae) on Parasite Prevalence and Intensity of Hesperomyces virescens (Ascomycota, Laboulbeniales)
Previous Article in Journal
Resistance to Permethrin, β-cyfluthrin, and Diazinon in Florida Horn Fly Populations
Previous Article in Special Issue
Host-Specific Activation of Entomopathogenic Nematode Infective Juveniles
Article Menu

Export Article

Open AccessArticle
Insects 2018, 9(2), 64; https://doi.org/10.3390/insects9020064

Within-Host Competition between Two Entomopathogenic Fungi and a Granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae)

1
Departamento de Entomologia e Acarologia, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo. Av. Pádua Dias, 11, C.P. 9, CEP 13418-900 Piracicaba, SP, Brazil
2
Embrapa Meio Ambiente, Rodovia SP-340, km 127.5, S/N—Tanquinho Velho, CEP 13820-000 Jaguariúna, SP, Brazil
3
Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C., Denmark
*
Author to whom correspondence should be addressed.
Received: 2 May 2018 / Revised: 30 May 2018 / Accepted: 1 June 2018 / Published: 13 June 2018
(This article belongs to the Special Issue Mechanisms Underlying Transmission of Insect Pathogens)
Full-Text   |   PDF [483 KB, uploaded 13 June 2018]   |  

Abstract

We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of Beauveria bassiana and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of Metarhizium anisopliae and B. bassiana, or M. anisopliae and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with M. anisopliae and DisaGV at the two highest inoculum rates. Co-infections with B. bassiana and M. anisopliae did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts. View Full-Text
Keywords: mixed infection; virulence; Beauveria bassiana; Metarhizium anisopliae; beta-baculovirus; sugarcane borer mixed infection; virulence; Beauveria bassiana; Metarhizium anisopliae; beta-baculovirus; sugarcane borer
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Pauli, G.; Moura Mascarin, G.; Eilenberg, J.; Delalibera Júnior, I. Within-Host Competition between Two Entomopathogenic Fungi and a Granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae). Insects 2018, 9, 64.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Insects EISSN 2075-4450 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top