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Open AccessFeature PaperCommunication
Insects 2017, 8(1), 27; doi:10.3390/insects8010027

Comparison of RNAi Sequences in Insect-Resistant Plants to Expressed Sequences of a Beneficial Lady Beetle: A Closer Look at Off-Target Considerations

Biological Control of Pests Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS 38776, USA
Academic Editor: Brian T. Forschler
Received: 21 October 2016 / Revised: 22 January 2017 / Accepted: 22 February 2017 / Published: 1 March 2017
View Full-Text   |   Download PDF [4361 KB, uploaded 1 March 2017]   |  

Abstract

Sequences obtained from transcriptomes of the lady beetle Coleomegilla maculata were compared to those designed for incorporation into crops. Searches of the transcriptomes identified sequences as the most likely to be closely similar to the sequences described in RNAi plant incorporated products. Some proposed prime RNAi pest management targets were also used to identify predicted orthologs from C. maculata. The lady beetle sequences were aligned with sequences from corn rootworms and Colorado potato beetles and, as appropriate in the case of targets, regions of similarity were compared with the genetic model organism for beetles, Tribolium castaneum. Some high levels of nucleotide identity were identified, particularly with an actin-derived sequence from Colorado potato beetle. This actin-derived sequence shared identical sequences with the lady beetle and a parasitic wasp. View Full-Text
Keywords: risk assessment; beneficial organism; genetic pest control; lady beetle risk assessment; beneficial organism; genetic pest control; lady beetle
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Allen, M.L. Comparison of RNAi Sequences in Insect-Resistant Plants to Expressed Sequences of a Beneficial Lady Beetle: A Closer Look at Off-Target Considerations. Insects 2017, 8, 27.

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