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Insects 2015, 6(2), 524-537; doi:10.3390/insects6020524

Identification of Eastern United States Reticulitermes Termite Species via PCR-RFLP, Assessed Using Training and Test Data

1
Department of Biology, University of Mississippi, Oxford, MS 38677, USA
2
Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Brian T. Forschler
Received: 17 April 2015 / Revised: 30 May 2015 / Accepted: 2 June 2015 / Published: 9 June 2015
View Full-Text   |   Download PDF [522 KB, uploaded 10 June 2015]   |  

Abstract

Reticulitermes termites play key roles in dead wood decomposition and nutrient cycling in forests. They also damage man-made structures, resulting in considerable economic loss. In the eastern United States, five species (R. flavipes, R. virginicus, R. nelsonae, R. hageni and R. malletei) have overlapping ranges and are difficult to distinguish morphologically. Here we present a molecular tool for species identification. It is based on polymerase chain reaction (PCR) amplification of a section of the mitochondrial cytochrome oxidase subunit II gene, followed by a three-enzyme restriction fragment length polymorphism (RFLP) assay, with banding patterns resolved via agarose gel electrophoresis. The assay was designed using a large set of training data obtained from a public DNA sequence database, then evaluated using an independent test panel of Reticulitermes from the Southern Appalachian Mountains, for which species assignments were determined via phylogenetic comparison to reference sequences. After refining the interpretive framework, the PCR-RFLP assay was shown to provide accurate identification of four co-occurring species (the fifth species, R. hageni, was absent from the test panel, so accuracy cannot yet be extended to training data). The assay is cost- and time-efficient, and will help improve knowledge of Reticulitermes species distributions. View Full-Text
Keywords: dead wood; Isoptera; mitochondrial DNA; molecular diagnostics dead wood; Isoptera; mitochondrial DNA; molecular diagnostics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Garrick, R.C.; Collins, B.D.; Yi, R.N.; Dyer, R.J.; Hyseni, C. Identification of Eastern United States Reticulitermes Termite Species via PCR-RFLP, Assessed Using Training and Test Data. Insects 2015, 6, 524-537.

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