Next Article in Journal
Biomechanical Properties of Hemlocks: A Novel Approach to Evaluating Physical Barriers of the Plant–Insect Interface and Resistance to a Phloem-Feeding Herbivore
Previous Article in Journal
Twenty Years of Elfin Enumeration: Abundance Patterns of Five Species of Callophrys (Lycaenidae) in Central Wisconsin, USA
Article Menu

Export Article

Open AccessArticle
Insects 2014, 5(2), 351-363; doi:10.3390/insects5020351

Glycolytic Activities in the Larval Digestive Tract of Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

Laboratory of Forest Zoology, Department of Forest Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan
Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan
Laboratory of Applied Biochemistry, Department of Bioresource Science, Junior College, Nihon University, Fujisawa, Kanagawa 252-0880, Japan
Author to whom correspondence should be addressed.
Received: 3 March 2014 / Revised: 17 April 2014 / Accepted: 24 April 2014 / Published: 5 May 2014
View Full-Text   |   Download PDF [673 KB, uploaded 5 May 2014]   |  


The larvae of the Japanese horned beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae: Dynastinae), are an example of a saprophage insect. Generally, Scarabaeid larvae, such as T. dichotomus, eat dead plant matter that has been broken down by fungi, such as Basidiomycota. It is thought that β-1,3-glucan, a constituent polysaccharide in microbes, is abundant in decayed plant matter. Studies of the degradation mechanism of β-1,3-glucan under these circumstances are lacking. In the current study, we sought to clarify the relationship between the capacity to degrade polysaccharides and the food habits of the larvae. The total activities and optimum pH levels of several polysaccharide-degrading enzymes from the larvae were investigated. The foregut, midgut and hindgut of final instar larvae were used. Enzymatic activities were detected against five polysaccharides (soluble starch, β-1,4-xylan, β-1,3-glucan, pectin and carboxymethyl cellulose) and four glycosides (p-nitrophenyl (PNP)-β-N-acetylglucosaminide, PNP-β-mannoside, PNP-β-glucoside and PNP-β-xyloside). Our results indicate that the digestive tract of the larvae is equipped with a full enzymatic system for degrading β-1,3-glucan and β-1,4-xylan to monomers. This finding elucidates the role of the polysaccharide-digesting enzymes in the larvae, and it is suggested that the larvae use these enzymes to enact their decomposition ability in the forest environment. View Full-Text
Keywords: enzyme; insect; polysaccharide; forest enzyme; insect; polysaccharide; forest

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wada, N.; Sunairi, M.; Anzai, H.; Iwata, R.; Yamane, A.; Nakajima, M. Glycolytic Activities in the Larval Digestive Tract of Trypoxylus dichotomus (Coleoptera: Scarabaeidae). Insects 2014, 5, 351-363.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Insects EISSN 2075-4450 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top