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Insects 2012, 3(3), 763-788; doi:10.3390/insects3030763
Review
Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management
Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Woodbridge Road, Menangle, NSW 2568, Australia
* Author to whom correspondence should be addressed.
Received: 13 June 2012; in revised form: 31 July 2012 / Accepted: 1 August 2012 / Published: 14 August 2012
(This article belongs to the Special Issue Augmentative Biological Control Using Parasitoids for Fruit Fly Management)
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Abstract: Parasitoid detection and identification is a necessary step in the development and implementation of fruit fly biological control strategies employing parasitoid augmentive release. In recent years, DNA-based methods have been used to identify natural enemies of pest species where morphological differentiation is problematic. Molecular techniques also offer a considerable advantage over traditional morphological methods of fruit fly and parasitoid discrimination as well as within-host parasitoid identification, which currently relies on dissection of immature parasitoids from the host, or lengthy and labour-intensive rearing methods. Here we review recent research focusing on the use of molecular strategies for fruit fly and parasitoid detection and differentiation and discuss the implications of these studies on fruit fly management.
Keywords: Diptera; Tephritidae; parasitoid; biological control; DNA barcode; PCR; microsatellites
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MDPI and ACS Style
Jenkins, C.; Chapman, T.A.; Micallef, J.L.; Reynolds, O.L. Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management. Insects 2012, 3, 763-788.
AMA StyleJenkins C, Chapman TA, Micallef JL, Reynolds OL. Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management. Insects. 2012; 3(3):763-788.
Chicago/Turabian StyleJenkins, Cheryl; Chapman, Toni A.; Micallef, Jessica L.; Reynolds, Olivia L. 2012. "Molecular Techniques for the Detection and Differentiation of Host and Parasitoid Species and the Implications for Fruit Fly Management." Insects 3, no. 3: 763-788.