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Diagnostics 2017, 7(3), 47; doi:10.3390/diagnostics7030047

Feasibility Study of an Optical Caustic Plasmonic Light Scattering Sensor for Human Serum Anti-Dengue Protein E Antibody Detection

1
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA
2
School of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA
3
Solexvintel S.A. de C.V., Ciudad de Mexico 01219, Mexico
4
Hospital Infantil de México Federico Gomez, Secretaria de Salud, Ciudad de Mexico 6720, Mexico
5
Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autonoma de Yucatán, Mérida 9709, Mexico
6
Hemolab Scientific S.A. de C.V., Merida 97100, Mexico
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 7 July 2017 / Revised: 4 August 2017 / Accepted: 11 August 2017 / Published: 17 August 2017
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Abstract

Antibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread of disease. However, most detection methods lack cost-effectiveness and field portability, which are essential features for achieving diagnosis in a timely manner. To address this, 3D-printed oblate spheroid sample chambers were fabricated to measure green light scattering of gold nanoparticles using an optical caustic focus to detect antibodies. Scattering signals of 20–200 nm gold nanoparticles using a green laser were compared to green light emitting diode (LED) light source signals and to Mie theory. The change in signal from 60 to 120 nm decreased in the order of Mie Theory > optical caustic scattering > 90° scattering. These results suggested that conjugating 60 nm gold nanoparticles and using an optical caustic system to detect plasmonic light scattering, would result in a sensitive test for detecting human antibodies in serum. Therefore, we studied the light scattering response of conjugated gold nanoparticles exposed to different concentrations of anti-protein E antibody, and a feasibility study of 10 human serum samples using dot blot and a handheld optical caustic-based sensor device. The overall agreement between detection methods suggests that the new sensor concept shows promise to detect gold nanoparticle aggregation in a homogeneous assay. Further testing and protocol optimization is needed to draw conclusions on the positive and negative predictive values for this new testing system. View Full-Text
Keywords: Dengue; gold nanoparticles; optical caustic; protein E; light scattering Dengue; gold nanoparticles; optical caustic; protein E; light scattering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

García, A.A.; Franco, L.S.; Pirez-Gomez, M.A.; Pech-Pacheco, J.L.; Mendez-Galvan, J.F.; Machain-Williams, C.; Talavera-Aguilar, L.; Espinosa-Carrillo, J.H.; Duarte-Villaseñor, M.M.; Be-Ortiz, C.; Espinosa-de los Monteros, L.E.; Castillo-Pacheco, A.; Garcia-Rejon, J.E. Feasibility Study of an Optical Caustic Plasmonic Light Scattering Sensor for Human Serum Anti-Dengue Protein E Antibody Detection. Diagnostics 2017, 7, 47.

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