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Life 2015, 5(1), 85-101; doi:10.3390/life5010085

Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada
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Author to whom correspondence should be addressed.
Academic Editors: Hans-Peter Klenk, Michael W. W. Adams and Roger A. Garrett
Received: 24 November 2014 / Accepted: 26 December 2014 / Published: 5 January 2015
(This article belongs to the Special Issue Archaea: Evolution, Physiology, and Molecular Biology)
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Abstract

Methanococcus maripaludis has two different surface appendages: type IV-like pili and archaella. Both structures are believed to be assembled using a bacterial type IV pilus mechanism. Each structure is composed of multiple subunits, either pilins or archaellins. Both pilins and archaellins are made initially as preproteins with type IV pilin-like signal peptides, which must be removed by a prepilin peptidase-like enzyme. This enzyme is FlaK for archaellins and EppA for pilins. In addition, both pilins and archaellins are modified with N-linked glycans. The archaellins possess an N-linked tetrasaccharide while the pilins have a pentasaccharide which consists of the archaellin tetrasaccharide but with an additional sugar, an unidentified hexose, attached to the linking sugar. In this report, we show that archaellins can be processed by FlaK in the absence of N-glycosylation and N-glycosylation can occur on archaellins that still retain their signal peptides. In contrast, pilins are not glycosylated unless they have been acted on by EppA to have the signal peptide removed. However, EppA can still remove signal peptides from non-glycosylated pilins. These findings indicate that there is a difference in the order of the posttranslational modifications of pilins and archaellins even though both are type IV pilin-like proteins. View Full-Text
Keywords: archaellins; pilins; signal peptide; N-linked glycosylation; archaea archaellins; pilins; signal peptide; N-linked glycosylation; archaea
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nair, D.B.; Jarrell, K.F. Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis. Life 2015, 5, 85-101.

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