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Life 2015, 5(1), 770-782; doi:10.3390/life5010770

Haloferax volcanii, as a Novel Tool for Producing Mammalian Olfactory Receptors Embedded in Archaeal Lipid Bilayer

1
Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari “Aldo Moro”, Piazza Giulio Cesare 11, I-70124 Bari, Italy
2
Institute for Chemical-Physical Processes, National Research Council (IPCF-CNR), Bari Unit, via Orabona 4, I-70126 Bari, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Hans-Peter Klenk, Michael W. W. Adams and Roger A. Garrett
Received: 14 January 2015 / Revised: 17 February 2015 / Accepted: 2 March 2015 / Published: 9 March 2015
(This article belongs to the Special Issue Archaea: Evolution, Physiology, and Molecular Biology)
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Abstract

The aim of this study was to explore the possibility of using an archaeal microorganism as a host system for expressing mammalian olfactory receptors (ORs). We have selected the archaeon Haloferax volcanii as a cell host system and one of the most extensively investigated OR, namely I7-OR, whose preferred ligands are short-chain aldehydes, such as octanal, heptanal, nonanal. A novel plasmid has been constructed to express the rat I7-OR, fused with a hexahistidine-tag for protein immunodetection. The presence of the recombinant receptor at a membrane level was demonstrated by immunoblot of the membranes isolated from the transgenic archaeal strain. In addition, the lipid composition of archaeonanosomes containing ORs has been characterized in detail by High-Performance Thin-Layer Chromatography (HPTLC) in combination with Matrix-Assisted Laser Desorption Ionization—Time-Of-Flight/Mass Spectrometry (MALDI-TOF/MS) analysis. View Full-Text
Keywords: extremely halophilic microorganisms; archaeal lipids; archaeonanosomes; olfactory receptors; biosensors extremely halophilic microorganisms; archaeal lipids; archaeonanosomes; olfactory receptors; biosensors
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lobasso, S.; Vitale, R.; Lopalco, P.; Corcelli, A. Haloferax volcanii, as a Novel Tool for Producing Mammalian Olfactory Receptors Embedded in Archaeal Lipid Bilayer. Life 2015, 5, 770-782.

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