Next Article in Journal / Special Issue
Appendages of the Cyanobacterial Cell
Previous Article in Journal
From Vesicles to Protocells: The Roles of Amphiphilic Molecules
Previous Article in Special Issue
Regulation of Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120 by a Group 2 Sigma Factor SigC
Article Menu

Export Article

Open AccessArticle
Life 2015, 5(1), 676-699; doi:10.3390/life5010676

Mechanisms of High Temperature Resistance of Synechocystis sp. PCC 6803: An Impact of Histidine Kinase 34

1
Department of Adaptation Biotechnologies, Global Change Research Centre, Academy of Sciences of the Czech Republic, Drásov 470, CZ-66424 Drásov, Czech Republic
2
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: John C. Meeks and Robert Haselkorn
Received: 14 November 2014 / Revised: 6 February 2015 / Accepted: 10 February 2015 / Published: 2 March 2015
(This article belongs to the Special Issue Cyanobacteria: Ecology, Physiology and Genetics)
View Full-Text   |   Download PDF [4166 KB, uploaded 2 March 2015]   |  

Abstract

Synechocystis sp. PCC 6803 is a widely used model cyanobacterium for studying responses and acclimation to different abiotic stresses. Changes in transcriptome, proteome, lipidome, and photosynthesis in response to short term heat stress are well studied in this organism, and histidine kinase 34 (Hik34) is shown to play an important role in mediating such response. Corresponding data on long term responses, however, are fragmentary and vary depending on parameters of experiments and methods of data collection, and thus are hard to compare. In order to elucidate how the early stress responses help cells to sustain long-term heat stress, as well as the role of Hik34 in prolonged acclimation, we examined the resistance to long-term heat stress of wild-type and ΔHik34 mutant of Synechocystis. In this work, we were able to precisely control the long term experimental conditions by cultivating Synechocystis in automated photobioreactors, measuring selected physiological parameters within a time range of minutes. In addition, morphological and ultrastructural changes in cells were analyzed and western blotting of individual proteins was used to study the heat stress-affected protein expression. We have shown that the majority of wild type cell population was able to recover after 24 h of cultivation at 44 °C. In contrast, while ΔHik34 mutant cells were resistant to heat stress within its first hours, they could not recover after 24 h long high temperature treatment. We demonstrated that the early induction of HspA expression and maintenance of high amount of other HSPs throughout the heat incubation is critical for successful adaptation to long-term stress. In addition, it appears that histidine kinase Hik34 is an essential component for the long term high temperature resistance. View Full-Text
Keywords: photosynthesis; pigments; ultrastructure; heat stress proteins; photobioreactor; cyanobacteria photosynthesis; pigments; ultrastructure; heat stress proteins; photobioreactor; cyanobacteria
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Červený, J.; Sinetova, M.A.; Zavřel, T.; Los, D.A. Mechanisms of High Temperature Resistance of Synechocystis sp. PCC 6803: An Impact of Histidine Kinase 34. Life 2015, 5, 676-699.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Life EISSN 2075-1729 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top