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Life 2014, 4(4), 770-787; doi:10.3390/life4040770

The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2

1
Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
2
Institute for Environmental Science and Technology, Saitama University, Saitama 338-8570, Japan
3
Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan
4
Core Research of Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan
*
Author to whom correspondence should be addressed.
Received: 16 August 2014 / Revised: 24 September 2014 / Accepted: 22 October 2014 / Published: 21 November 2014
(This article belongs to the Special Issue Cyanobacteria: Ecology, Physiology and Genetics)
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Abstract

The cyAbrB2 transcriptional regulator is essential for active sugar catabolism in Synechocystis sp. PCC 6803 grown under light conditions. In the light-grown cyabrB2-disrupted mutant, glycogen granules and sugar phosphates corresponding to early steps in the glycolytic pathway accumulated to higher levels than those in the wild-type (WT) strain, whereas the amounts of 3-phosphoglycerate, phosphoenolpyruvate and ribulose 1,5-bisphosphate were significantly lower. We further determined that accumulated glycogen granules in the mutant could be actively catabolized under dark conditions. Differences in metabolite levels between WT and the mutant became less substantial during dark incubation due to a general quantitative decrease in metabolite levels. Notable exceptions, however, were increases in 2-oxoglutarate, histidine, ornithine and citrulline in the WT but not in the mutant. The amounts of cyAbrBs were highly responsive to the availability of light both in transcript and protein levels. When grown under light-dark cycle conditions, diurnal oscillatory pattern of glycogen content of the mutant was lost after the second dark period. These observations indicate that cyAbrB2 is dispensable for activation of sugar catabolism under dark conditions but involved in the proper switching between day and night metabolisms. View Full-Text
Keywords: capillary electrophoresis-mass spectrometry; cyAbrB2; dark; glycogen; metabolic regulation; Synechocystis capillary electrophoresis-mass spectrometry; cyAbrB2; dark; glycogen; metabolic regulation; Synechocystis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hanai, M.; Sato, Y.; Miyagi, A.; Kawai-Yamada, M.; Tanaka, K.; Kaneko, Y.; Nishiyama, Y.; Hihara, Y. The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2 . Life 2014, 4, 770-787.

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