Life 2013, 3(3), 375-385; doi:10.3390/life3030375
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Domain Structures and Inter-Domain Interactions Defining the Holoenzyme Architecture of Archaeal D-Family DNA Polymerase

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Received: 15 April 2013; in revised form: 26 June 2013 / Accepted: 27 June 2013 / Published: 5 July 2013
(This article belongs to the Special Issue Extremophiles and Extreme Environments)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Archaea-specific D-family DNA polymerase (PolD) forms a dimeric heterodimer consisting of two large polymerase subunits and two small exonuclease subunits. According to the protein-protein interactions identified among the domains of large and small subunits of PolD, a symmetrical model for the domain topology of the PolD holoenzyme is proposed. The experimental evidence supports various aspects of the model. The conserved amphipathic nature of the N-terminal putative α-helix of the large subunit plays a key role in the homodimeric assembly and the self-cyclization of the large subunit and is deeply involved in the archaeal PolD stability and activity. We also discuss the evolutional transformation from archaeal D-family to eukaryotic B-family polymerase on the basis of the structural information.
Keywords: D-family DNA polymerase; DNA replication; binding domain; molecular structure; hyperthermophilic archaea; Pyrococcus
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MDPI and ACS Style

Matsui, I.; Matsui, E.; Yamasaki, K.; Yokoyama, H. Domain Structures and Inter-Domain Interactions Defining the Holoenzyme Architecture of Archaeal D-Family DNA Polymerase. Life 2013, 3, 375-385.

AMA Style

Matsui I, Matsui E, Yamasaki K, Yokoyama H. Domain Structures and Inter-Domain Interactions Defining the Holoenzyme Architecture of Archaeal D-Family DNA Polymerase. Life. 2013; 3(3):375-385.

Chicago/Turabian Style

Matsui, Ikuo; Matsui, Eriko; Yamasaki, Kazuhiko; Yokoyama, Hideshi. 2013. "Domain Structures and Inter-Domain Interactions Defining the Holoenzyme Architecture of Archaeal D-Family DNA Polymerase." Life 3, no. 3: 375-385.

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