Next Article in Journal
Benthic Diatoms of the Ying River (Huaihe River Basin, China) and Their Application in Water Trophic Status Assessment
Previous Article in Journal
Water Compensation and Its Implication of the Three Gorges Reservoir for the River-Lake System in the Middle Yangtze River, China
Article Menu

Export Article

Open AccessArticle
Water 2018, 10(8), 1012; https://doi.org/10.3390/w10081012

Legionella Detection in Environmental Samples as an Example for Successful Implementation of qPCR

Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, 8010 Graz, Austria
*
Author to whom correspondence should be addressed.
Received: 21 June 2018 / Revised: 27 July 2018 / Accepted: 27 July 2018 / Published: 31 July 2018
(This article belongs to the Section Water Quality and Ecosystems)
View Full-Text   |   Download PDF [483 KB, uploaded 31 July 2018]   |  

Abstract

Waterborne diseases are a serious threat because of their ability to infect a high number of individuals in a short time span, such as during outbreaks of Legionellosis. This significantly highlights the need for the rapid detection and quantification of bacteria in environmental water samples. The aim of this study was to investigate the feasibility of quantitative Polymerase Chain Reaction (qPCR) for the detection of Legionellapneumophila (L. pneumophila) in environmental water samples and comparison of standard culture methods for Legionella detection with qPCR. Our study reached a negative predictive value (NPV) for L. pneumophila of 80.7% and for L. pneumophila serogroup 1 (sg1) the calculated NPV was 87.0%. The positive predictive value (PPV) for L. pneumophila was 53.9% and for L. pneumophila sg1 PPV was 21.4%. Results showed a correlation between qPCR and culture with an R2 value of 0.8973 for L. pneumophila, whereas no correlation was observed for the detection of L. pneumophila sg1. In our study, qPCR proved useful for the identification of L. pneumophila negative samples. However, despite the obvious benefits (sample handling, rapid generation of results), qPCR needs to be improved regarding the PPV before it can replace culture in water quality assessment. View Full-Text
Keywords: Legionella; qPCR; culture; cooling towers; L. pneumophila; drinking water Legionella; qPCR; culture; cooling towers; L. pneumophila; drinking water
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Toplitsch, D.; Platzer, S.; Pfeifer, B.; Hautz, J.; Mascher, F.; Kittinger, C. Legionella Detection in Environmental Samples as an Example for Successful Implementation of qPCR. Water 2018, 10, 1012.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Water EISSN 2073-4441 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top