Next Article in Journal
Groundwater Quality Assessment: An Improved Approach to K-Means Clustering, Principal Component Analysis and Spatial Analysis: A Case Study
Next Article in Special Issue
Antibiotic Resistance and Extended-Spectrum Beta-Lactamase Production of Escherichia coli Isolated from Irrigation Waters in Selected Urban Farms in Metro Manila, Philippines
Previous Article in Journal
Analysis of the Long-term Precipitation Trend in Illinois and Its Implications for Agricultural Production
Previous Article in Special Issue
Distribution and Abundance of Antibiotic Resistance Genes in Sand Settling Reservoirs and Drinking Water Treatment Plants across the Yellow River, China
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Water 2018, 10(4), 436; https://doi.org/10.3390/w10040436

An Increase of Abundance and Transcriptional Activity for Acinetobacter junii Post Wastewater Treatment

1
Water Desalination and Reuse Center (WDRC), Biological and Environmental Science & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia
2
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
*
Author to whom correspondence should be addressed.
Received: 29 January 2018 / Revised: 23 March 2018 / Accepted: 28 March 2018 / Published: 6 April 2018
(This article belongs to the Special Issue Antimicrobial Resistance in Environmental Waters)
View Full-Text   |   Download PDF [27690 KB, uploaded 3 May 2018]   |  

Abstract

A membrane bioreactor (MBR)-based wastewater treatment plant (WWTP) in Saudi Arabia is assessed over a five-month period in 2015 and once in 2017 for bacterial diversity and transcriptional activity using metagenomics, metatranscriptomics and real time quantitative polymerase chain reaction (RT-qPCR). Acinetobacter spp. are shown to be enriched in the chlorinated effluent. Members of the Acinetobacter genus are the most abundant in the effluent and chlorinated effluent. At the species level, Acinetobacter junii have higher relative abundances post MBR and chlorination. RNA-seq analysis show that, in A. junii, 288 genes and 378 genes are significantly upregulated in the effluent and chlorinated effluent, respectively, with 98 genes being upregulated in both. RT-qPCR of samples in 2015 and 2017 confirm the upregulation observed in RNA-seq. Analysis of the 98 genes show that majority of the upregulated genes are involved in cellular repair and metabolism followed by resistance, virulence, and signaling. Additionally, two different subpopulations of A. junii are observed in the effluent and chlorinated effluent. The upregulation of cellular repair and metabolism genes, and the formation of different subpopulations of A. junii in both effluents provide insights into the mechanisms employed by A. junii to persist in the conditions of a WWTP. View Full-Text
Keywords: Acinetobacter junii; wastewater treatment plant; antibiotic resistance; metal resistance genes; persistence Acinetobacter junii; wastewater treatment plant; antibiotic resistance; metal resistance genes; persistence
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Jumat, M.R.; Haroon, M.F.; Al-Jassim, N.; Cheng, H.; Hong, P.-Y. An Increase of Abundance and Transcriptional Activity for Acinetobacter junii Post Wastewater Treatment. Water 2018, 10, 436.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Water EISSN 2073-4441 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top