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Genes 2018, 9(8), 406; https://doi.org/10.3390/genes9080406

Nitrogen Supply and Leaf Age Affect the Expression of TaGS1 or TaGS2 Driven by a Constitutive Promoter in Transgenic Tobacco

1
Collaborative Innovation Center of Henan Grain Crops, Henan Agriculture University, Zhengzhou 450000, China
2
Department of Biochemistry and Molecular Biology, College of Life Science, Henan Agriculture University, Zhengzhou 450000, China
3
State Key Laboratory of Wheat and Maize Crop Science in China, Henan Agriculture University, Zhengzhou 450000, China
*
Author to whom correspondence should be addressed.
Received: 5 July 2018 / Revised: 1 August 2018 / Accepted: 7 August 2018 / Published: 10 August 2018
(This article belongs to the Special Issue Improving Plant Nutrient Use efficiency)
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Abstract

Glutamine synthetase (GS) plays a key role in nitrogen metabolism. Here, two types of tobacco transformants, overexpressing Triticum aestivum GS1 (TaGS1) or GS2 (TaGS2), were analysed. Four independent transformed lines, GS1-TR1, GS1-TR2, GS2-TR1 and GS2-TR2, were used for the nitrogen treatment. Under nitrogen-sufficient conditions, the leaves of GS2-TR showed high accumulation of the TaGS2 transcript, while those of GS1-TR showed a low TaGS1 transcript levels. However, compared with nitrogen-sufficient conditions, the TaGS1 transcript level increased in the leaves under nitrogen starvation, but the TaGS2 transcript level decreased. In addition, the TaGS1 and TaGS2 transcript levels were highest in the middle leaves under nitrogen-sufficient and starvation conditions. These results show that nitrogen supply and leaf age regulate TaGS expression, even when they are driven by a super-promoter. Additionally, in regard to nitrogen metabolism level, the lower leaves of the GS1-TR exhibited lower NH4+ and higher amino acid contents, while the upper leaves exhibited higher amino acid, soluble protein and chlorophyll contents. The leaves of the GS2-TR exhibited lower NH4+ but higher amino acid, soluble protein and chlorophyll contents. Given the role that GS isoforms play in nitrogen metabolism, these data suggest that TaGS1 overexpression may improve nitrogen transport, and that TaGS2 overexpression may improve nitrogen assimilation under nitrogen stress. View Full-Text
Keywords: tobacco; overexpression; glutamine synthetase 1; glutamine synthetase 2; expression regulation; nitrogen metabolism tobacco; overexpression; glutamine synthetase 1; glutamine synthetase 2; expression regulation; nitrogen metabolism
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Wei, Y.; Shi, A.; Jia, X.; Zhang, Z.; Ma, X.; Gu, M.; Meng, X.; Wang, X. Nitrogen Supply and Leaf Age Affect the Expression of TaGS1 or TaGS2 Driven by a Constitutive Promoter in Transgenic Tobacco. Genes 2018, 9, 406.

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