Next Article in Journal
The Unexplored Diversity of Pleolipoviruses: The Surprising Case of Two Viruses with Identical Major Structural Modules
Previous Article in Journal
Characterizing the DNA Methyltransferases of Haloferax volcanii via Bioinformatics, Gene Deletion, and SMRT Sequencing
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Genes 2018, 9(3), 130; https://doi.org/10.3390/genes9030130

De Novo Transcriptome Assembly and Characterization of the Synthesis Genes of Bioactive Constituents in Abelmoschus esculentus (L.) Moench

1
Vegetable Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2
Key Labortatory of Creative Agricultrue, Ministry of Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3
College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China
*
Author to whom correspondence should be addressed.
Received: 12 January 2018 / Revised: 15 February 2018 / Accepted: 19 February 2018 / Published: 27 February 2018
(This article belongs to the Section Plant Genetics and Genomics)
Full-Text   |   PDF [10778 KB, uploaded 27 February 2018]   |  

Abstract

Abelmoschus esculentus (okra or lady’s fingers) is a vegetable with high nutritional value, as well as having certain medicinal effects. It is widely used as food, in the food industry, and in herbal medicinal products, but also as an ornamental, in animal feed, and in other commercial sectors. Okra is rich in bioactive compounds, such as flavonoids, polysaccharides, polyphenols, caffeine, and pectin. In the present study, the concentrations of total flavonoids and polysaccharides in five organs of okra were determined and compared. Transcriptome sequencing was used to explore the biosynthesis pathways associated with the active constituents in okra. Transcriptome sequencing of five organs (roots, stem, leaves, flowers, and fruits) of okra enabled us to obtain 293,971 unigenes, of which 232,490 were annotated. Unigenes related to the enzymes involved in the flavonoid biosynthetic pathway or in fructose and mannose metabolism were identified, based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. All of the transcriptional datasets were uploaded to Sequence Read Archive (SRA). In summary, our comprehensive analysis provides important information at the molecular level about the flavonoid and polysaccharide biosynthesis pathways in okra. View Full-Text
Keywords: flavonoids; glycosyltransferase; polysaccharide; okra; transcriptome flavonoids; glycosyltransferase; polysaccharide; okra; transcriptome
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Zhang, C.; Dong, W.; Gen, W.; Xu, B.; Shen, C.; Yu, C. De Novo Transcriptome Assembly and Characterization of the Synthesis Genes of Bioactive Constituents in Abelmoschus esculentus (L.) Moench. Genes 2018, 9, 130.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top