Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes
AbstractA lipolytic screening with fungal strains isolated from lignocellulosic waste collected in banana plantation dumps was carried out. A Trichoderma harzianum strain (B13-1) showed good extracellular lipolytic activity (205 UmL−1). Subsequently, functional screening of the lipolytic activity on Rhodamine B enriched with olive oil as the only carbon source was performed. The successful growth of the strain allows us to suggest that a true lipase is responsible for the lipolytic activity in the B13-1 strain. In order to identify the gene(s) encoding the protein responsible for the lipolytic activity, in silico identification and characterization of triacylglycerol lipases from T. harzianum is reported for the first time. A survey in the genome of this fungus retrieved 50 lipases; however, bioinformatic analyses and putative functional descriptions in different databases allowed us to choose seven lipases as candidates. Suitability of the bioinformatic screening to select the candidates was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The gene codifying 526309 was expressed when the fungus grew in a medium with olive oil as carbon source. This protein shares homology with commercial lipases, making it a candidate for further applications. The success in identifying a lipase gene inducible with olive oil and the suitability of the functional screening and bioinformatic survey carried out herein, support the premise that the strategy can be used in other microorganisms with sequenced genomes to search for true lipases, or other enzymes belonging to large protein families. View Full-Text
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Description: Table S1. List of primers used in this study. Table S2. List of all lipase proteins identified in the T. harzianum genome homepage. Figure S1. Fungal strains B13-1 (A) and B13-3 (B) selected in screening for extracellular lipolytic activity using olive oil as carbon source in Rhodamine B test. Figure S2. Three-dimensional model proposed for (A) 551811; (B) 87496; (C) 526309; (D) 514427; (E) 510832; (F) 92423; (G) 135964; (H) 77338; (I) 514252; (J) 78181. In all protein models: (1) Red, -helixes; yellow, -strands. (2) Close up showing the lid (yellow), the catalytic triad (in red) and the oxyanion (in blue). (3) Superposition with best template protein model (see Table 4). (4) Superposition of catalytic triad in both proteins. All models were generated by I-TASSER and visualizations were performed in PyMOL. Figure S2. Continued.
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Canseco-Pérez, M.A.; Castillo-Avila, G.M.; Chi-Manzanero, B.; Islas-Flores, I.; Apolinar-Hernández, M.M.; Rivera-Muñoz, G.; Gamboa-Angulo, M.; Sanchez-Teyer, F.; Couoh-Uicab, Y.; Canto-Canché, B. Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes. Genes 2018, 9, 62.
Canseco-Pérez MA, Castillo-Avila GM, Chi-Manzanero B, Islas-Flores I, Apolinar-Hernández MM, Rivera-Muñoz G, Gamboa-Angulo M, Sanchez-Teyer F, Couoh-Uicab Y, Canto-Canché B. Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes. Genes. 2018; 9(2):62.Chicago/Turabian Style
Canseco-Pérez, Miguel A.; Castillo-Avila, Genny M.; Chi-Manzanero, Bartolomé; Islas-Flores, Ignacio; Apolinar-Hernández, Max M.; Rivera-Muñoz, Gerardo; Gamboa-Angulo, Marcela; Sanchez-Teyer, Felipe; Couoh-Uicab, Yeny; Canto-Canché, Blondy. 2018. "Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes." Genes 9, no. 2: 62.
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