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Genes 2017, 8(9), 218; doi:10.3390/genes8090218

3D Microstructure Inhibits Mesenchymal Stem Cells Homing to the Site of Liver Cancer Cells on a Microchip

1,†
,
1,†
,
1
,
1,2,3
and
1,2,*
1
Materials Genome Institute, Shanghai University, Shanghai 200444, China
2
Biomedical Research Institute, Shenzhen Peking University—The Hong Kong University of Science and Technology Medical Center, Shenzhen 518000, China
3
Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 1 August 2017 / Revised: 28 August 2017 / Accepted: 29 August 2017 / Published: 1 September 2017
(This article belongs to the Special Issue From the Lab-on-a-Chip to the Organ-on-a-Chip)
View Full-Text   |   Download PDF [5920 KB, uploaded 1 September 2017]   |  

Abstract

The cell microenvironment consists of multiple types of biophysical and biochemical factors, and represents a complex integrated system that is variable in both time and space. Studies show that changes in biochemical and biophysical factors in cell microenvironments result in significant changes in cellular forms and functions, especially for stem cells. Mesenchymal stem cells (MSCs) are derived from adult stem cells of the mesoderm and play an important role in tissue engineering, regenerative medicine and even cancer therapy. Furthermore, it is found that MSCs can interact with multiple types of tumor cells. The interaction is reflected as two totally different aspects. The negative aspect is that MSCs manifest as tumor-associated fibroblasts and could induce migration of cancer cells and promote tumor formation. On the other hand, MSCs can home to sites of the tumor microenvironment, directionally migrate toward tumor cells and cause tumor cell apoptosis. In this study, we designed and made a simple microfluidic chip for cell co-culture, and studied stem cell homing behavior in the interaction between MSCs and liver cancer cells. Moreover, by etching a three-dimensional microstructure on the base and adding transforming growth factor-β (TGF-β) in the co-culture environment, we studied the impact of biophysical and biochemical factors on stem cell homing behavior, and the causes of such impact. View Full-Text
Keywords: microchip; mesenchymal stem cells; cell homing; cell co-culture; microstructure microchip; mesenchymal stem cells; cell homing; cell co-culture; microstructure
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yang, X.; Xu, X.; Zhang, Y.; Wen, W.; Gao, X. 3D Microstructure Inhibits Mesenchymal Stem Cells Homing to the Site of Liver Cancer Cells on a Microchip. Genes 2017, 8, 218.

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