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Genes 2017, 8(9), 215; doi:10.3390/genes8090215

Intrachromosomal Rearrangements in Rodents from the Perspective of Comparative Region-Specific Painting

1
Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia
2
Synthetic Biological Unit, Novosibirsk State University, 630090 Novosibirsk, Russia
3
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071 Moscow, Russia
4
Zoological Institute, Russian Academy of Sciences, 199034 Saint-Petersburg, Russia
5
Department of Biology, Anthropology Laboratories, University of Florence, 50122 Florence, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Liehr
Received: 11 July 2017 / Revised: 22 August 2017 / Accepted: 23 August 2017 / Published: 30 August 2017
(This article belongs to the Special Issue Chromosomal Evolution)
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Abstract

It has long been hypothesized that chromosomal rearrangements play a central role in different evolutionary processes, particularly in speciation and adaptation. Interchromosomal rearrangements have been extensively mapped using chromosome painting. However, intrachromosomal rearrangements have only been described using molecular cytogenetics in a limited number of mammals, including a few rodent species. This situation is unfortunate because intrachromosomal rearrangements are more abundant than interchromosomal rearrangements and probably contain essential phylogenomic information. Significant progress in the detection of intrachromosomal rearrangement is now possible, due to recent advances in molecular biology and bioinformatics. We investigated the level of intrachromosomal rearrangement in the Arvicolinae subfamily, a species-rich taxon characterized by very high rate of karyotype evolution. We made a set of region specific probes by microdissection for a single syntenic region represented by the p-arm of chromosome 1 of Alexandromys oeconomus, and hybridized the probes onto the chromosomes of four arvicolines (Microtus agrestis, Microtus arvalis, Myodes rutilus, and Dicrostonyx torquatus). These experiments allowed us to show the intrachromosomal rearrangements in the subfamily at a significantly higher level of resolution than previously described. We found a number of paracentric inversions in the karyotypes of M. agrestis and M. rutilus, as well as multiple inversions and a centromere shift in the karyotype of M. arvalis. We propose that during karyotype evolution, arvicolines underwent a significant number of complex intrachromosomal rearrangements that were not previously detected. View Full-Text
Keywords: centromere shift; chromosome painting; comparative cytogenetics; fluorescence in situ hybridization; inversion; microdissection; small mammals; voles centromere shift; chromosome painting; comparative cytogenetics; fluorescence in situ hybridization; inversion; microdissection; small mammals; voles
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Romanenko, S.A.; Serdyukova, N.A.; Perelman, P.L.; Pavlova, S.V.; Bulatova, N.S.; Golenishchev, F.N.; Stanyon, R.; Graphodatsky, A.S. Intrachromosomal Rearrangements in Rodents from the Perspective of Comparative Region-Specific Painting. Genes 2017, 8, 215.

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