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Genes 2017, 8(9), 212; doi:10.3390/genes8090212

Chloroplast Genome Sequence of Clusterbean (Cyamopsis tetragonoloba L.): Genome Structure and Comparative Analysis

1
ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012, India
2
Division of Vegetable Sciences, Indian Agricultural Research Institute, Pusa, New Delhi 110012, India
3
National Agri-Food Biotechnology Institute (NABI), Mohali, Punjab 140306, India
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Aureliano Bombarely
Received: 18 July 2017 / Revised: 16 August 2017 / Accepted: 21 August 2017 / Published: 19 September 2017
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

Clusterbean (Cyamopsis tetragonoloba L.), also known as guar, belongs to the family Leguminosae, and is an annual herbaceous legume. Guar is the main source of galactomannan for gas mining industries. In the present study, the draft chloroplast genome of clusterbean was generated and compared to some of the previously reported legume chloroplast genomes. The chloroplast genome of clusterbean is 152,530 bp in length, with a quadripartite structure consisting of large single copy (LSC) and small single copy (SSC) of 83,025 bp and 17,879 bp in size, respectively, and a pair of inverted repeats (IRs) of 25,790 bp in size. The chloroplast genome contains 114 unique genes, which includes 78 protein coding genes, 30 tRNAs, 4 rRNAs genes, and 2 pseudogenes. It also harbors a 50 kb inversion, typical of the Leguminosae family. The IR region of the clusterbean chloroplast genome has undergone an expansion, and hence, the whole rps19 gene is included in the IR, as compared to other legume plastid genomes. A total of 220 simple sequence repeats (SSRs) were detected in the clusterbean plastid genome. The analysis of the clusterbean plastid genome will provide useful insights for evolutionary, molecular and genetic engineering studies. View Full-Text
Keywords: clusterbean; Leguminosae; chloroplast genome; Illumina Hiseq 1000 platform; codon usage; microsatellites clusterbean; Leguminosae; chloroplast genome; Illumina Hiseq 1000 platform; codon usage; microsatellites
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Kaila, T.; Chaduvla, P.K.; Rawal, H.C.; Saxena, S.; Tyagi, A.; Mithra, S.V.A.; Solanke, A.U.; Kalia, P.; Sharma, T.R.; Singh, N.K.; Gaikwad, K. Chloroplast Genome Sequence of Clusterbean (Cyamopsis tetragonoloba L.): Genome Structure and Comparative Analysis. Genes 2017, 8, 212.

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