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Genes 2017, 8(2), 61; doi:10.3390/genes8020061

In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia

Laboratory of Organic Polymer Chemistry, LCPO, UMR 5629 CNRS, University of Bordeaux, Bordeaux-INP, Pessac 33600, France
Molecular Imaging and Innovative Therapies in Oncology, IMOTION, EA 7435, University of Bordeaux, 146 rue Léo Saignat, case 127, Bordeaux cedex 33076, France
Department of Electricity and Electronics, University of the Basque Country (UPV/EHU), P.K. 644, Leioa 48940, Spain
Institute for Condensed Matter Chemistry of Bordeaux, ICMCB, UPR 9048, CNRS, University of Bordeaux, Pessac F-33600 France
Authors to whom correspondence should be addressed.
Academic Editor: Selvarangan Ponnazhagan
Received: 22 November 2016 / Revised: 16 January 2017 / Accepted: 1 February 2017 / Published: 8 February 2017
(This article belongs to the Section Human Genomics and Genetic Diseases)
View Full-Text   |   Download PDF [2916 KB, uploaded 8 February 2017]   |  


The present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and noninvasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected subcutaneously with Matrigel™ to generate so-called pseudotumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 h after heating. We showed that dextran-coated magnetic iron oxide nanoparticle dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as noninvasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release. View Full-Text
Keywords: magnetic hyperthermia; gene therapies; heat shock protein promoter; in vivo optical imaging; magnetic polymer-coated nanoparticles magnetic hyperthermia; gene therapies; heat shock protein promoter; in vivo optical imaging; magnetic polymer-coated nanoparticles

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sandre, O.; Genevois, C.; Garaio, E.; Adumeau, L.; Mornet, S.; Couillaud, F. In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia. Genes 2017, 8, 61.

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