Next Article in Journal
Mechanisms of Post-Replication DNA Repair
Previous Article in Journal
Altered Intracellular Milieu of ADAR2-Deficient Motor Neurons in Amyotrophic Lateral Sclerosis
Article Menu

Export Article

Open AccessArticle
Genes 2017, 8(2), 61; doi:10.3390/genes8020061

In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia

1
Laboratory of Organic Polymer Chemistry, LCPO, UMR 5629 CNRS, University of Bordeaux, Bordeaux-INP, Pessac 33600, France
2
Molecular Imaging and Innovative Therapies in Oncology, IMOTION, EA 7435, University of Bordeaux, 146 rue Léo Saignat, case 127, Bordeaux cedex 33076, France
3
Department of Electricity and Electronics, University of the Basque Country (UPV/EHU), P.K. 644, Leioa 48940, Spain
4
Institute for Condensed Matter Chemistry of Bordeaux, ICMCB, UPR 9048, CNRS, University of Bordeaux, Pessac F-33600 France
*
Authors to whom correspondence should be addressed.
Academic Editor: Selvarangan Ponnazhagan
Received: 22 November 2016 / Revised: 16 January 2017 / Accepted: 1 February 2017 / Published: 8 February 2017
(This article belongs to the Section Human Genomics and Genetic Diseases)
View Full-Text   |   Download PDF [2916 KB, uploaded 8 February 2017]   |  

Abstract

The present work aims to demonstrate that colloidal dispersions of magnetic iron oxide nanoparticles stabilized with dextran macromolecules placed in an alternating magnetic field can not only produce heat, but also that these particles could be used in vivo for local and noninvasive deposition of a thermal dose sufficient to trigger thermo-induced gene expression. Iron oxide nanoparticles were first characterized in vitro on a bio-inspired setup, and then they were assayed in vivo using a transgenic mouse strain expressing the luciferase reporter gene under transcriptional control of a thermosensitive promoter. Iron oxide nanoparticles dispersions were applied topically on the mouse skin or injected subcutaneously with Matrigel™ to generate so-called pseudotumors. Temperature was monitored continuously with a feedback loop to control the power of the magnetic field generator and to avoid overheating. Thermo-induced luciferase expression was followed by bioluminescence imaging 6 h after heating. We showed that dextran-coated magnetic iron oxide nanoparticle dispersions were able to induce in vivo mild hyperthermia compatible with thermo-induced gene expression in surrounding tissues and without impairing cell viability. These data open new therapeutic perspectives for using mild magnetic hyperthermia as noninvasive modulation of tumor microenvironment by local thermo-induced gene expression or drug release. View Full-Text
Keywords: magnetic hyperthermia; gene therapies; heat shock protein promoter; in vivo optical imaging; magnetic polymer-coated nanoparticles magnetic hyperthermia; gene therapies; heat shock protein promoter; in vivo optical imaging; magnetic polymer-coated nanoparticles
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sandre, O.; Genevois, C.; Garaio, E.; Adumeau, L.; Mornet, S.; Couillaud, F. In Vivo Imaging of Local Gene Expression Induced by Magnetic Hyperthermia. Genes 2017, 8, 61.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top