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Genes 2017, 8(11), 333; doi:10.3390/genes8110333

Differentially Expressed tRNA-Derived Small RNAs Co-Sediment Primarily with Non-Polysomal Fractions in Drosophila

1
Department of Molecular Biology and Genetics, İzmir Institute of Technology, Gülbahçeköyü, 35430 Urla İzmir, Turkey
2
Unit of Molecular Cell Biology, Johannes Gutenberg-University Mainz, Bentzelweg 3, 55128 Mainz, Germany
3
German Center for Neurodegenerative Diseases Dresden, DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence, TU Dresden FetscherstraBe 105, 01307 Dresden, Germany
*
Author to whom correspondence should be addressed.
Received: 24 October 2017 / Revised: 15 November 2017 / Accepted: 16 November 2017 / Published: 20 November 2017
(This article belongs to the Special Issue Non-coding RNAs)
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Abstract

Recent studies point to the existence of poorly characterized small regulatory RNAs generated from mRNAs, rRNAs and tRNAs. To explore the subcellular location of tRNA-derived small RNAs, 0–1 and 7–8 h Drosophila embryos were fractionated on sucrose density gradients. Analysis of 12,553,921 deep-sequencing reads from unfractionated and fractionated Drosophila embryos has revealed that tRFs, which are detected mainly from the 5’ends of tRNAs, co-sediment with the non-polysomal fractions. Interestingly, the expression levels of a subset of tRFs change temporally following the maternal-to-zygotic transition in embryos. We detected non-polysomal association of tRFs in S2 cells as well. Differential tRF expression pattern points to developmental significance at the organismal level. These results suggest that tRFs are associated primarily with the non-polysomal complexes in Drosophila embryos and S2 cells. View Full-Text
Keywords: tRF; tRNA; polysome; Drosophila tRF; tRNA; polysome; Drosophila
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Göktaş, Ç.; Yiğit, H.; Coşacak, M.İ.; Akgül, B. Differentially Expressed tRNA-Derived Small RNAs Co-Sediment Primarily with Non-Polysomal Fractions in Drosophila. Genes 2017, 8, 333.

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