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Genes 2017, 8(11), 305; doi:10.3390/genes8110305

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

1
Department of Biology and Biotechnology ‘L. Spallanzani’, via Ferrata 9, 27100 Pavia, Italy
2
Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB-UNL), Avenida da República, EAN, 2780-157 Oeiras, Portugal
Present Address: Viral Control of Cellular Pathways and Biology of Tumorigenesis Unit, European Institute of Oncology (IFOM-IEO), via Adamello 16, 20139 Milano, Italy
*
Author to whom correspondence should be addressed.
Received: 1 September 2017 / Revised: 24 October 2017 / Accepted: 24 October 2017 / Published: 3 November 2017
(This article belongs to the Special Issue Genetic Regulation of Abiotic Stress Responses)
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Abstract

Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is involved in DNA repair pathways as it mends the topoisomerase I—DNA covalent complexes. In plants, a small Tdp1 gene family, composed by Tdp1α and Tdp1β genes, was identified, but the roles of these genes in abiotic stress responses are not fully understood. To investigate their specific stress response patterns, the present study made use of bioinformatic and molecular tools to look into the Tdp1β gene function, so far described only in the plant kingdom, and compare it with Tdp1α gene coding for the canonical, highly conserved α isoform. The expression profiles of Tdp1α and Tdp1β genes were examined under abiotic stress conditions (cold, heat, high osmolarity, salt, and UV-B) in two model species, Arabidopsis thaliana and Medicago truncatula. The two isoforms of topoisomerase I (TOP1α and TOP1β) were also taken into consideration in view of their known roles in DNA metabolism and cell proliferation. Data relative to gene expression in Arabidopsis were retrieved from the AtGenExpress microarray dataset, while quantitative Real-Time PCR was carried out to evaluate the stress response in M. truncatula cell cultures. These analyses revealed that Tdp1β gene expression was enhanced during the first hour of treatment, whereas Tdp1α enhanced expression succeeded at subsequent timepoints. In agreement with the gene-specific responses to abiotic stress conditions, the promoter regions of Tdp1α and Tdp1β genes are well equipped with stress-related cis-elements. An in-depth bioinformatic characterization of the HIRAN motif, a distinctive feature of the Tdp1β protein, showed its wide distribution in chromatin remodeling and DNA repair proteins. The reported data suggests that Tdp1β functions in the early response to abiotic stresses. View Full-Text
Keywords: abiotic stress; Arabidopsis thaliana; HIRAN domain; Medicago truncatula; tyrosyl-DNA phosphodiesterase abiotic stress; Arabidopsis thaliana; HIRAN domain; Medicago truncatula; tyrosyl-DNA phosphodiesterase
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MDPI and ACS Style

Sabatini, M.E.; Pagano, A.; Araùjo, S.; Balestrazzi, A.; Macovei, A. The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses. Genes 2017, 8, 305.

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