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Genes 2017, 8(10), 289; doi:10.3390/genes8100289

Identification of Gene Expression Changes in the Aorta of ApoE Null Mice Fed a High-Fat Diet

1
Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou 510515, China
2
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
3
Department of Cardiology, Huqiao Medical Center, Nanfang Hospital, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou 510515, China
*
Author to whom correspondence should be addressed.
Received: 31 August 2017 / Revised: 8 October 2017 / Accepted: 16 October 2017 / Published: 24 October 2017
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Abstract

Atherosclerosis is a chronic multifactorial inflammatory disease with high worldwide prevalence, and has become the leading cause of death. In the present study, we analyzed global gene expression changes in the aorta of Apolipoprotein E (ApoE) null mice fed a high-fat diet by using RNA-seq. We identified a total of 280 differentially expressed genes, of which 163 genes were upregulated and 117 genes were downregulated by high-fat diet compared with normal diet. Functional clustering and gene network analysis revealed that fatty acid metabolic process is crucial for atherosclerosis. By examining of the promoter regions of differentially expressed genes, we identified four causal transcription factors. Additionally, through connectivity map (CMap) analysis, multiple compounds were identified to have anti-atherosclerotic effects due to their ability to reverse gene expression during atherosclerosis. Our study provides a valuable resource for in-depth understanding of the mechanism underlying atherosclerosis. View Full-Text
Keywords: ApoE null mice; RNA-seq; atherosclerosis ApoE null mice; RNA-seq; atherosclerosis
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Xi, D.; Zhao, J.; Zhao, M.; Fu, W.; Guo, Z.; Chen, H. Identification of Gene Expression Changes in the Aorta of ApoE Null Mice Fed a High-Fat Diet. Genes 2017, 8, 289.

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