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Erratum published on 3 March 2017, see Genes 2017, 8(3), 91.

Open AccessArticle
Genes 2017, 8(1), 18; doi:10.3390/genes8010018

Error-Free Bypass of 7,8-dihydro-8-oxo-2′-deoxyguanosine by DNA Polymerase of Pseudomonas aeruginosa Phage PaP1

1
College of Pharmacy and Bioengineering, Chongqing University of Technology, No. 69 Hongguang Street, Banan District, Chongqing 400054, China
2
Public Health Laboratory Sciences and Toxicology, West China School of Public Health, Sichuan University, No. 17 People’s South Road, Chengdu 610041, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Eishi Noguchi
Received: 21 November 2016 / Revised: 26 December 2016 / Accepted: 30 December 2016 / Published: 6 January 2017
(This article belongs to the Special Issue DNA Replication Controls)
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Abstract

As one of the most common forms of oxidative DNA damage, 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxoG) generally leads to G:C to T:A mutagenesis. To study DNA replication encountering 8-oxoG by the sole DNA polymerase (Gp90) of Pseudomonas aeruginosa phage PaP1, we performed steady-state and pre-steady-state kinetic analyses of nucleotide incorporation opposite 8-oxoG by Gp90 D234A that lacks exonuclease activities on ssDNA and dsDNA substrates. Gp90 D234A could bypass 8-oxoG in an error-free manner, preferentially incorporate dCTP opposite 8-oxoG, and yield similar misincorporation frequency to unmodified G. Gp90 D234A could extend beyond C:8-oxoG or A:8-oxoG base pairs with the same efficiency. dCTP incorporation opposite G and dCTP or dATP incorporation opposite 8-oxoG showed fast burst phases. The burst of incorporation efficiency (kpol/Kd,dNTP) is decreased as dCTP:G > dCTP:8-oxoG > dATP:8-oxoG. The presence of 8-oxoG in DNA does not affect its binding to Gp90 D234A in a binary complex but it does affect it in a ternary complex with dNTP and Mg2+, and dATP misincorporation opposite 8-oxoG further weakens the binding of Gp90 D234A to DNA. This study reveals Gp90 D234A can bypass 8-oxoG in an error-free manner, providing further understanding in DNA replication encountering oxidation lesion for P.aeruginosa phage PaP1. View Full-Text
Keywords: P. aeruginosa phage PaP1; DNA polymerase; 8-oxoG; steady-state kinetics; pre-steady-state kinetics; nucleotide incorporation P. aeruginosa phage PaP1; DNA polymerase; 8-oxoG; steady-state kinetics; pre-steady-state kinetics; nucleotide incorporation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gu, S.; Xue, Q.; Liu, Q.; Xiong, M.; Wang, W.; Zhang, H. Error-Free Bypass of 7,8-dihydro-8-oxo-2′-deoxyguanosine by DNA Polymerase of Pseudomonas aeruginosa Phage PaP1. Genes 2017, 8, 18.

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