Genes 2016, 7(2), 8; doi:10.3390/genes7020008
Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus
Di-An Fang 1,2,†,*
, Jin-Rong Duan 1,†, Yan-Feng Zhou 1,†, Min-Ying Zhang 1, Dong-Po Xu 1,2, Kai Liu 1 and Pao Xu 1,*
, Jin-Rong Duan 1,†, Yan-Feng Zhou 1,†, Min-Ying Zhang 1, Dong-Po Xu 1,2, Kai Liu 1 and Pao Xu 1,*
1
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China
2
Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Xuejiali 69, Wuxi 214128, China
â€
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 24 September 2015 / Revised: 14 January 2016 / Accepted: 14 January 2016 / Published: 28 January 2016
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
Abstract
Heat shock proteins play essential roles in basic cellular events. Spawning migration is a complex process, with significant structural and biochemical changes taking place in the adult gonad. To date, the molecular mechanisms underlying migration reproductive biology remain undetermined. In this regard, a full length HSP90AA1 comprising 2608 nucleotides from the anadromous fish Coilia nasus was characterized, encoding 742 amino acid (aa) residues with potential phosphorylation sites. HSP90AA1 mRNA transcripts were detected in all organs, especially in the gonad. Furthermore, the greatest transcript levels were found during the developmental phase, while the lowest levels were found during the resting phase. In addition, the strongest immunolabeling positive signal was found in the primary spermatocyte and oocyte, with lower positive staining in secondary germ cells, and a weak or absent level in the mature sperm and oocyte. Interestingly, HSP90AA1 was mainly located in the cytoplasm of germ cells. These results are important for understanding the molecular mechanism of anadromous migration reproductive biology. In combination with data from other fish species, the result of this present study may facilitate further investigations on the spawning migration mechanism. View Full-Text
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Scifeed alert for new publications
Never miss any articles matching your research from any publisher- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Share & Cite This Article
MDPI and ACS Style
Fang, D.-A.; Duan, J.-R.; Zhou, Y.-F.; Zhang, M.-Y.; Xu, D.-P.; Liu, K.; Xu, P. Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus. Genes 2016, 7, 8.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.