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Genes 2016, 7(2), 8; doi:10.3390/genes7020008

Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus

1,2,†,* , 1,†
,
1,†
,
1
,
1,2
,
1
and
1,*
1
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214128, China
2
Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Xuejiali 69, Wuxi 214128, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 24 September 2015 / Revised: 14 January 2016 / Accepted: 14 January 2016 / Published: 28 January 2016
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
View Full-Text   |   Download PDF [2180 KB, uploaded 28 January 2016]   |  

Abstract

Heat shock proteins play essential roles in basic cellular events. Spawning migration is a complex process, with significant structural and biochemical changes taking place in the adult gonad. To date, the molecular mechanisms underlying migration reproductive biology remain undetermined. In this regard, a full length HSP90AA1 comprising 2608 nucleotides from the anadromous fish Coilia nasus was characterized, encoding 742 amino acid (aa) residues with potential phosphorylation sites. HSP90AA1 mRNA transcripts were detected in all organs, especially in the gonad. Furthermore, the greatest transcript levels were found during the developmental phase, while the lowest levels were found during the resting phase. In addition, the strongest immunolabeling positive signal was found in the primary spermatocyte and oocyte, with lower positive staining in secondary germ cells, and a weak or absent level in the mature sperm and oocyte. Interestingly, HSP90AA1 was mainly located in the cytoplasm of germ cells. These results are important for understanding the molecular mechanism of anadromous migration reproductive biology. In combination with data from other fish species, the result of this present study may facilitate further investigations on the spawning migration mechanism. View Full-Text
Keywords: Coilia nasus; HSP90AA1 expression; anadromous fish; migration mechanism Coilia nasus; HSP90AA1 expression; anadromous fish; migration mechanism
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Fang, D.-A.; Duan, J.-R.; Zhou, Y.-F.; Zhang, M.-Y.; Xu, D.-P.; Liu, K.; Xu, P. Molecular Characteristic, Protein Distribution and Potential Regulation of HSP90AA1 in the Anadromous Fish Coilia nasus. Genes 2016, 7, 8.

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