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Genes 2016, 7(12), 118; doi:10.3390/genes7120118

New Oligonucleotide Probes for ND-FISH Analysis to Identify Barley Chromosomes and to Investigate Polymorphisms of Wheat Chromosomes

1,2,†
,
1,†
,
1
,
1,* and 1,2,*
1
Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Wenjiang, Chengdu 611130, China
2
Institute of Ecological Agriculture, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Lukas Mueller
Received: 23 July 2016 / Revised: 21 October 2016 / Accepted: 28 November 2016 / Published: 5 December 2016
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
View Full-Text   |   Download PDF [2061 KB, uploaded 5 December 2016]   |  

Abstract

Oligonucleotide probes that can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) analysis are convenient tools for identifying chromosomes of wheat (Triticum aestivum L.) and its relatives. New oligonucleotide probes, Oligo-HvT01, Oligo-pTa71-1, Oligo-s120.1, Oligo-s120.2, Oligo-s120.3, Oligo-275.1, Oligo-275.2, Oligo-k566 and Oligo-713, were designed based on the repetitive sequences HVT01, pTa71, pTa-s120, pTa-275, pTa-k566 and pTa-713. All these probes can be used for ND-FISH analysis and some of them can be used to detect polymorphisms of wheat chromosomes. Probes Oligo-HvT01, Oligo-pTa71-1, Oligo-s120.3, Oligo-275.1, Oligo-k566 and Oligo-713 can, respectively, replace the roles of their original sequences to identify chromosomes of some barley (Hordeum vulgare ssp. vulgare) and the common wheat variety Chinese Spring. Oligo-s120.1, Oligo-s120.2 and Oligo-275.2 produced different hybridization patterns from the ones generated by their original sequences. In addition, Oligo-s120.1, Oligo-s120.2 and Oligo-s120.3, which were derived from pTa-s120, revealed different signal patterns. Likewise, Oligo-275.1 and Oligo-275.2, which were derived from pTa-275, also displayed different hybridization patterns. These results imply that differently arranged or altered structural statuses of tandem repeats might exist on different chromosome regions. These new oligonucleotide probes provide extra convenience for identifying some wheat and barley chromosomes, and they can display polymorphisms of wheat chromosomes. View Full-Text
Keywords: barley; wheat; ND-FISH; chromosomal polymorphism; tandem repeats barley; wheat; ND-FISH; chromosomal polymorphism; tandem repeats
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MDPI and ACS Style

Tang, S.; Qiu, L.; Xiao, Z.; Fu, S.; Tang, Z. New Oligonucleotide Probes for ND-FISH Analysis to Identify Barley Chromosomes and to Investigate Polymorphisms of Wheat Chromosomes. Genes 2016, 7, 118.

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