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Genes 2016, 7(10), 91; doi:10.3390/genes7100091

Identification, Characterization and Expression of Methuselah-Like Genes in Dastarcus helophoroides (Coleoptera: Bothrideridae)

1
Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling 712100, China
2
Institute of Biotechnology, Shaanxi Xueqian Normal University, Xi’an 710100, China
These authors contributed equally to this study.
*
Author to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 13 March 2016 / Revised: 15 October 2016 / Accepted: 17 October 2016 / Published: 21 October 2016
(This article belongs to the Section Molecular Genetics)
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Abstract

Dastarcus helophoroides, which has a relatively longer lifespan compared to other insects, is one of the most effective natural enemies of many large-body long-horned beetles. Methuselah (Mth) is associated with the lifespan, stress resistance, and reproduction in Drosophila melanogaster, but Mth is not present in non-drosophiline insects. A number of methuselah-like genes (mth-likes, mthls) have been identified in non-drosophiline insects, but it is still unknown whether they are present in Dastarcus helophoroides. We identified three novel mth-like genes in D. helophoroides: mth-like1, mth-like2, and mth-like5, and carried out bioinformatic analysis based on the full-length nucleic acid sequences and deduced amino acid sequences. Real-time quantitative polymerase chain reaction (RT-qPCR) showed variations in expression patterns of mth-like genes in different tissues (highly expressed in reproductive systems) and at different developmental stages, indicating that mth-likes were likely be involved in reproduction and development. The altered mRNA expression in aging adults and under oxidation, high temperature, and starvation stress, indicated that mth-like genes were likely to be involved in aging and the resistance of oxidation, high temperature, and starvation. These results characterize, for the first time, the basic properties of three mth-like genes from D. helophoroides that probably play important roles in development, aging, reproduction, and stress resistance. View Full-Text
Keywords: Dastarcus helophoroides; mth-like genes; expression profile; development; lifespan; reproduction; stress resistance Dastarcus helophoroides; mth-like genes; expression profile; development; lifespan; reproduction; stress resistance
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MDPI and ACS Style

Zhang, Z.; Wang, H.; Hao, C.; Zhang, W.; Yang, M.; Chang, Y.; Li, M. Identification, Characterization and Expression of Methuselah-Like Genes in Dastarcus helophoroides (Coleoptera: Bothrideridae). Genes 2016, 7, 91.

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