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Genes 2015, 6(2), 436-450; doi:10.3390/genes6020436

Arrest of Viral Proliferation by Ectopic Copies of Its Cognate Replication Origin

1
Department of Biochemistry and Cancer Biology, School of Medicine, Meharry Medical College, 1005 D.B. Todd Jr. Boulevard, Nashville, TN 37208, USA
2
Department of Obstetrics and Gynecology, School of Medicine, Meharry Medical College, 1005 D.B. Todd Jr. Boulevard, Nashville, TN 37208, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Peter Frank
Received: 7 April 2015 / Revised: 10 June 2015 / Accepted: 18 June 2015 / Published: 23 June 2015
(This article belongs to the Special Issue DNA Replication)
View Full-Text   |   Download PDF [1103 KB, uploaded 23 June 2015]   |  

Abstract

The initiation step of DNA replication is the crucial determinant of proliferation in all organisms. This step depends on the specific interaction of DNA sequences present at origins of DNA replication and their cognate activators. We wished to explore the hypothesis that the presence of ectopic origin copies may interfere with proper genome duplication. Bacteriophage λ was used as a model system. To this end, the outcome of an infection of an E. coli strain harboring ectopic copies of the λ origin region was analyzed. By measuring the effect on the host growth, viral production, and electro-microscopic visualization of the resulting λ replicative intermediates, we concluded that the ectopic copies had prevented the normal initiation step of λ DNA replication. These results suggest that DNA decoys encoding viral origins could constitute effective tools to specifically arrest viral proliferation. View Full-Text
Keywords: viral propagation arrest; λ phage; ectopic origins of DNA replication; DNA replication; DNA electron microscopy viral propagation arrest; λ phage; ectopic origins of DNA replication; DNA replication; DNA electron microscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Valenzuela, M.S.; Sharan, C. Arrest of Viral Proliferation by Ectopic Copies of Its Cognate Replication Origin. Genes 2015, 6, 436-450.

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