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Genes 2013, 4(3), 388-434; doi:10.3390/genes4030388
Published: 19 August 2013
Abstract: Checkpoints monitor critical cell cycle events such as chromosome duplication and segregation. They are highly conserved mechanisms that prevent progression into the next phase of the cell cycle when cells are unable to accomplish the previous event properly. During S phase, cells also provide a surveillance mechanism called the DNA replication checkpoint, which consists of a conserved kinase cascade that is provoked by insults that block or slow down replication forks. The DNA replication checkpoint is crucial for maintaining genome stability, because replication forks become vulnerable to collapse when they encounter obstacles such as nucleotide adducts, nicks, RNA-DNA hybrids, or stable protein-DNA complexes. These can be exogenously induced or can arise from endogenous cellular activity. Here, we summarize the initiation and transduction of the replication checkpoint as well as its targets, which coordinate cell cycle events and DNA replication fork stability.
Dividing cells go through cycles of cell growth, DNA replication, chromatin condensation, chromosome segregation, and cell division. All these highly complicated processes need to be accurately performed and tightly controlled in order to produce viable offspring. Checkpoints were initially characterized as mechanisms that ensure that a certain process is started only after the previous one has been successfully completed . Originally, yeast genetic studies identified checkpoint genes that ensured cell survival and delayed the onset of mitosis during DNA replication arrest, yet many of these factors are now known to be involved in the DNA damage response [2,3,4]. Thus, in addition to preserving the order of events, checkpoints factors contribute to the maintenance of genome stability . The loss of genome stability has extremely deleterious consequences, including malignant transformation or cell death .
Cells are exposed to many types of exogenous and endogenous stress that can modify or damage the DNA. These include oxidative stress, ionizing or UV-light mediated irradiation, and chemical damage, as well as inherent DNA compaction, tightly bound DNA binding proteins, and transcription. Even excessive or premature initiation or replication can cause replicative stress , arguing that normal DNA metabolism itself can interfere with DNA replication . This is particularly true in cells with perturbed G1/S control, such as oncogene-transformed cells. In response to excessive DNA damage, wild-type cells arrest the cell cycle in G1 phase, before starting DNA replication, or in G2 phase before entering mitosis . On the other hand, replication fork-associated damage provokes a response that delays progression through S phase, by controlling forks and initiation events [10,11,12].
At the heart of all DNA damage checkpoint responses, are the two kinases Tel1 (telomere maintenance 1; ATM in mammals, see Table 1 for an overview of checkpoint protein names in several model organisms) and Mec1 (Mitosis Entry Checkpoint 1; ATR in mammals) (Figure 1) . Both are phosphoinositide 3-kinase (PI3K)-related kinases (PIKKs) that share significant sequence homology and phosphorylate an overlapping set of substrates. Both show a preference for serine or threonine residues followed by glutamine ([S/T]Q) or a hydrophobic residue [13,14,15,16]. Often these target sites are found in SQ/TQ cluster domains (SCDs) . All PIKKs, not only Mec1 and Tel1, share a common domain architecture in which the kinase domain is flanked by both FRAPP, ATM, TRRAP (FAT), and FAT C-terminal (FATC) domains (Figure 2A), all being conserved alpha-helical regions [18,19]. Because FAT and FATC domains are always present in combination, it has been suggested that these two domains interact with each other, potentially providing a scaffold or binding sites for other proteins .
In budding yeast, Mec1 is active even in an unperturbed S phase, as it can regulate dNTP levels and replication initiation without blocking cell cycle progression [20,21]. ATRMec1 becomes hyperactivated in response to a wide variety of DNA insults and is essential for cell viability, whereas ATMTel1 is activated primarily by double-strand breaks (DSBs) and its loss in budding yeast is not lethal. Nonetheless, in mammalian cells, mutation of either homolog leads to an elevated predisposition towards cancer . Once localized to the site of DNA damage and activated by DNA damage sensing proteins, either kinase can initiate a signaling cascade that transduces the signal through mediator proteins Mrc1 and Rad9 (Claspin, BRCA1, MDC1 and 53BP1 in mammals) to the effector kinases Rad53 and Chk1 (CHK2 and CHK1 in mammals) (Figure 1) [22,23,24,25]. Effector kinases are transiently recruited to sites of DNA damage and are released after their activation [26,27], allowing transmission of the checkpoint response to a range of effector proteins . In addition to the effector kinases, Mec1 and Tel1 also phosphorylate proteins bound at sites of damage, such as budding yeast histone H2A (the H2AX variant in mammals), generating γH2AX, to provoke local chromatin changes .
|Table 1. Conserved checkpoint proteins and their functions.|
|S. cerevisiae||S. pombe||H. sapiens||Function|
|Rad24-RFC||Rad17-RFC||RAD17-RFC||RFC-like complex, 9-1-1 clamp loader|
|Ddc1-Rad17-Mec3||Rad9-Rad1-Hus1||RAD9-RAD1-HUS1||9-1-1 complex, DNA damage checkpoint clamp, Mec1 activation|
|Dpb11||Cut5/Rad4||TOPBP1||Mec1 ATR activation|
|Dna2||Dna2||DNA2||Mec1 activation in S phase|
|Mre11-Rad50-Xrs2||Mre11/Rad32-Rad50-Nbs1||MRE11-RAD50-NBS1||MRX/MRN complex, DSB resection, Tel1/ATM recruitment|
|Mec1-Ddc2||Rad3-Rad26||ATR-ATRIP||checkpoint signaling kinase|
|Tel1||Tel1||ATM||checkpoint signaling kinase|
|Mrc1||Mrc1||Claspin||fork-associated, checkpoint mediator|
|Rad9||Crb2||53BP1, BRCA1||checkpoint mediator|
|Sgs1||Rqh1||BLM, WRN||fork-associated, Rad53 activation|
Gene name abbreviations: Rad24 (radiation sensitive 24), RFC (replication factor c), Ddc1 (DNA damage checkpoint 1), Mec3 (Mitosis entry checkpoint 3), Hus1 (hydroxyurea sensitive 1), Dpb11 (DNA polymerase B 11), Cut5 (cell untimely torn 5), TOPBP1 (DNA topoisomerase 2 binding protein 1), Dna2 (DNA synthesis defective 2), Mre11(meiotic recombination 11), Xrs2 (X-ray sensitive 2), Nbs1 (Nijmegen breakage syndrome 1), ATR (ATM and Rad3-related), ATRIP (ATR interacting protein), Tel1 (telomere maintenance 1), ATM (Ataxia telangiectasia mutated), Mrc1 (mediator of the replication checkpoint 1), Crb2 (cut5 repeat binding 2), 53BP1 (tumor suppressor p53 binding protein 1), BRCA1 (breast cancer 1, early-onset), Sgs1 (slow growth suppressor 1), rqh1 (RecQ-type DNA helicase 1), BLM (Bloom syndrome protein), WRN (Werner syndrome ATP-dependent helicase), Cds1 (checking DNA synthesis 1), CHK2 (checkpoint kinase 2), CHK1 (checkpoint kinase 1).
DNA damage occurs in all stages of the cell cycle, yet cells are particularly vulnerable to insults during DNA replication, when the double helix is unwound. Indeed, in S phase, defects in one strand can have serious consequences on the integrity of the daughter chromosome. Moreover, the single-stranded DNA (ssDNA) that is generated during replication, is intrinsically more labile than double-stranded (dsDNA) . Consistently, sites that slow the DNA replication fork have been shown to correlate with sites of enhanced genome fragility . To cope with this danger, cells provide a surveillance mechanism called intra-S-phase or DNA replication checkpoint (Figure 1A). This checkpoint slows genome replication by inhibiting the firing of late origins [10,11], and protects stalled replication forks by preventing their conversion to DSBs and/or reducing recombination events [32,33,34]. Consistently, it has been shown that the loss of replication checkpoint factors provokes high levels of spontaneous gross chromosomal rearrangements in budding yeast . The factors involved in this checkpoint are highly conserved and many, including ATR itself, have tumor suppressor roles in mammals .
Here we review recent findings on the replication checkpoint. We will first discuss the nature of the DNA lesions that provoke a checkpoint response. We then describe the mechanism of ATRMec1 activation and summarize the functions served by the replication checkpoint, especially with respect to replication fork stability. We will discuss how cells downregulate the checkpoint signal to resume the cell cycle after the insult has been removed, and finally examine the coordination between two checkpoint PIKK kinases, ATRMec1 and ATMTel1. Although we focus primarily on insights from studies in budding yeast, we relate those findings to results obtained from other organisms.
2. Replication Checkpoint Initiation
2.1. Lesions that Activate the Checkpoint
Replication forks themselves play a critical role in inducing a checkpoint signal. Only when a critical number of replication forks initiate and encounter lesions, will the replication checkpoint signal become robust [34,36]. This has seeded the notion of a threshold for activation of the replication checkpoint. After treatment with a replication stress-inducing drug (hydroxyurea, HU), long stretches of ssDNA (about 200 nucleotides) are exposed at stalled forks . These extended stretches of ssDNA themselves contribute to the induction of the checkpoint response, but they are not sufficient: a double-stranded primer with a free 5' end is also required . The ds-ssDNA junction structure can arise from a variety of replication and repair processes, such as lagging strand DNA synthesis, nucleotide excision repair , or from resection at DSBs. This structure is recognized by the 9-1-1 checkpoint clamp and its loading factor (see below and Figure 1A,B). At DSBs ATMTel1 is recruited and activated initially by the Mre11-Rad50-Nbs1Xrs2 complex, which then promotes resection. Resection generates ssDNA and a ds-ssDNA junction, which in turn activate ATRMec1 [39,40,41,42] (see also Section 6). Both at resected DSBs and at stalled replication forks, ssDNA is rapidly coated with the trimeric ssDNA binding complex RPA (replication protein A) . RPA-bound ssDNA interacts with ATRIPDdc2, an essential cofactor of the ATRMec1 kinase [44,45] (Figure 1). Mutations in RPA that disrupt its interaction with ATRIPDdc2 reduce checkpoint activation [44,46,47,48]. In budding yeast, the RPA-Ddc2 interaction also requires Mec1, suggesting either that there may be an independent RPA binding surface on Mec1, or that Mec1 changes Ddc2 conformation in a way that favors RPA interaction .
The ds-ssDNA junctions are recognized by the Rad24-RFC complex that loads the 9-1-1 checkpoint clamp (Ddc1, Rad17 and Mec3 in S. cerevisiae). In vitro analysis argues that 9-1-1 can be loaded at both 3' and 5' junctions, although if RPA is bound to the ssDNA, the 9-1-1 complex prefers to load at 5' junctions. These structures, together with RPA, are sufficient to activate the ATRMec1 checkpoint in a cell-free system [37,50]. In budding yeast, Mec1 phosphorylates the Ddc1 subunit (human RAD9) of the 9-1-1 complex, which can then recruit Dpb11 (human TopBP1). TopBP1Dpb11 further stimulates ATRMec1 kinase activity [51,52,53,54,55,56,57]. This indicates that in addition to its loading onto ssDNA, ATRMec1-ATRIPDdc2 needs to contact an activator in order to induce the checkpoint response (see Section 2.3). In addition to this, several studies have suggested that mismatch repair factors at the site of DNA damage provide an alternative means to recruit and activate ATRMec1 [58,59,60].
2.2. Drugs Used to Induce and Study Checkpoint Responses
As mentioned above, special DNA structures initiate the ATRMec1 checkpoint response. To study the replication checkpoint and downstream responses in vivo, a variety of DNA-damaging and fork stalling agents are used, and these provoke the checkpoint response in different ways (summarized in Table 2). Natural replication fork stalling can also occur, generally due to secondary DNA structures (e.g., G-quadruplexes), RNA-DNA hybrids found at genes, tightly bound transcription complexes (e.g., at rDNA or tRNA genes) or specific protein-DNA complexes, like that formed by the replication fork barrier protein (Fob1), which prevents forks from colliding with RNA PolI in the rDNA repeats [61,62]. Natural fork pausing, however, does not provoke a global checkpoint response. We note that the proteins involved in a chemically induced checkpoint responses depend on the type of damage induced and can vary with the dose applied, as demonstrated recently for camptothecin . Further complexity in the checkpoint network stems from differences in cell type or genetic background. For example, the checkpoint response is more prone to be activated in cells deficient for DNA repair, such the rad18 mutant, which is deficient for post-replication repair [64,65]. Other lesions that expose primed ssDNA and activate the replication checkpoint arise when replicative helicase and polymerase functions are uncoupled  (Figure 3A,B). Here we summarize commonly used treatments that induce the checkpoint response, and highlight differences in the responses they elicit.
|Table 2. S. cerevisiae checkpoint responses differ, depending on the treatment.|
|Treatment/Impediments||Mode of action||Result||Responders ( S.cerevisiae)|
|hydroxyurea (HU)||inhibits ribonucleotide reductase—dNTP pools become depleted||uncoupling of helicase and polymerase function; ssDNA is exposed||Mec1, Mrc1, Sgs1|
|aphidicolin||inhibits DNA polymerases||uncoupling of helicase and polymerase function; ssDNA is exposed||Mec1, Mrc1|
|methylmethanesulfonate (MMS)||alkylates DNA||uncoupling of helicase and polymerase function; ssDNA is exposed; in addition DNA repair takes place, that also leads to ssDNA; requires replication forks to induce checkpoint response||Mec1, Rad9 (Mrc1, Sgs1)|
|ultraviolet light/4-NQO||induces Thymidine dimerization||induces DNA repair, that leads to ssDNA||Mec1, Rad9 Mrc1|
|crosslinking agents (cisplatin/nitrogen mustard)||causes DNA inter-strand crosslinks||both helicase and polymerase are blocked; in addition DNA repair takes place, that also leads to ssDNA||Mec1/Tel1, Rad9|
|ionizing irradiation (IR)/bleomycin||causes single and double strand breaks||breaks are directly recognized by MRX-Tel1; resection leads to ssDNA||Mec1/Tel1; Rad9|
|camptothecin (CPT)||inhibits Topoisomerase I, keeps it in a DNA-bound confirmation||both helicase and polymerase are blocked; double strand breaks are actively induced by DNA repair machinery||Mec1/Tel1, Rad9|
|natural fork barriers (rDNA, t-RNA genes, transcription||slow down replisome progression||both helicase and polymerase are slowed down||-|
One commonly used means to trigger the replication checkpoint is to treat cells with either hydroxyurea (HU) or aphidicolin. HU inhibits ribonucleotide reductase (RNR) by reducing the reactive tyrosyl radical in the active center of the enzyme . When replication is initiated, dNTP pools are rapidly depleted if RNR is inhibited , and this leads to a stalling of DNA polymerases. Aphidicolin, on the other hand, directly inhibits DNA polymerases  without affecting the replicative helicase . Accordingly, polymerases are blocked, but MCM helicases continue to move, generating long stretches of ssDNA that trigger the replication checkpoint .
Interstrand crosslinks (ICs), such as those caused by cisplatin, do the opposite: they tend to block the MCM helicase in front of the replication fork. There is no uncoupling of helicase and polymerase, and hence no immediate activation of the replication checkpoint. However, often the replication forks pause 20–40 nucleotides before reaching the IC lesion, and often structure-specific nucleases will process the template or nascent strand, generating ssDNA [71,72], which in turn leads to checkpoint activation.
Methyl methanesulfonate (MMS) creates bulky lesions by alkylating DNA. Alkylation alone does not elicit the checkpoint response, but requires that the replication fork collides with the DNA adduct. Therefore, MMS-induced checkpoint responses are restricted to S-phase cells . Although both MMS and HU activate an S-phase checkpoint, it is important to note that they do not provoke equivalent responses [24,46,73,74]. In response to MMS, cells activate several repair pathways, including base excision repair (BER), DNA damage tolerance pathways such as trans-lesion synthesis , and homologous recombination (HR). However, since DSBs are not detected on MMS, it is not clear whether it is the repair process itself, or an uncoupling of leading and lagging strand synthesis, which provokes what appears to be a combined replication/DNA damage checkpoint response .
Ionizing radiation (IR) or treatment with bleomycin or its derivatives (e.g., Zeocin®) cause DSBs, which activate the DNA damage response initially through the ATMTel1 kinase, and after processing, through ATRMec1 . It should be kept in mind that IR or bleomycin derivatives induce oxidative damage and single-strand nicks much more efficiently than DSBs. At sufficiently high doses, UV light also provokes an ATRMec1-dependent response , for the pyrimidine dimers caused by UV treatment are recognized by the nucleotide excision repair (NER) machinery, which itself creates a ssDNA patch during the repair process . While these lead to ATRMec1 activation, the pathway involves Rad9 (homologue of 53BP1 or BRCA1), a mediator typically implicated at DSBs . In mammals, Mre11-Rad50-Nbs1Xrs2 (MRN) nuclease has been shown to be involved in UV-dependent ATRMec1 activation, possibly again by creating a single-strand patch .
Camptothecin inhibits topoisomerase I (Top1) by blocking religation after the enzyme has made a ssDNA nick and becomes covalently linked to the DNA end . These structures arrest replication forks and are cytotoxic , yet they only provoke a mild checkpoint response , presumably because the lesion is not accompanied by helicase-polymerase uncoupling nor extensive resection. The so-called replication run-off model  has indicated that replication forks running into these ssDNA nicks are converted into DSBs, which generally depend on a recombination-dependent mechanism for replication fork restart [63,84]. Recent data indicate that torsional stress generated by Top1 inhibition may lead to fork slowing, and suggest that the formation of a DSB is an active process involving cleavage by the endonuclease Mus81. An artificial system that generates a similar lesion uses a mutant form of the site-specific Flp recombinase to generate a covalent protein-DNA complex, adjacent to a ssDNA nick . This lesion, like the Top1-camptothecin complex, does not induce a checkpoint response in wild-type yeast, but recruits the recombination machinery for repair following collision with a replication fork (L. Bjergbaek, personal communication). This illustrates the broad range of responses elicited by exogenous agents, and underscores the importance of highlighting the type of damaging agent used.
2.3. Mec1/ATR Activation
An accumulation of RPA-coated ssDNA recruits ATRMec1-ATRIPDdc2, just like a DSB bound by MRNMRX recruits ATMTel1. However, whereas MRNMRX also activates ATMTel1, ssDNA-RPA is not sufficient to induce ATRMec1 activation. As discussed above, ds-ssDNA junctions that recruit the 9-1-1 checkpoint clamp are also required for activation (see Section 2.1). In budding yeast, Ddc1, a subunit of the 9-1-1 complex that binds the ds-ssDNA junction, has been shown to be capable of activating Mec1 alone under low salt conditions in vitro [86,87], just as the artificial juxtaposition of multiple Ddc1 and Ddc2 molecules can activate Mec1 in vivo . In higher organisms, on the other hand, RAD9Ddc1 instead creates a binding site for the ATRMec1 activator TopBP1Dpb11 [51,52,53]. TopBP1Dpb11 contains eight BRCA1 C-terminal (BRCT) domains (Figure 2A) and interacts with phosphorylated RAD9Ddc1 through its BRCT domains 1 and 2. In addition, the MRNMRX complex has been shown to recruit TopBP1Dpb11 through its BRCT domains 3-6 (Figure 2B) . Overexpression of a domain of TopBP1Dpb11 that sits between its BRCT motifs 6 and 7 (called AAD for ATR activation domain), also leads to ATRMec1 activation. Indeed, one can bypass the need for the intact 9-1-1 clamp by tethering the AAD to PCNA or histone H2B [51,90]. TopBP1Dpb11 itself binds to ATRIPDdc2, and mutations within its TopBP1Dpb11 binding region can block ATRMec1 activation. Finally, a region of ATRMec1, between the kinase and the FATC domain, is important for TopBP1Dpb11-mediated ATRMec1 activation  (Figure 2A,B). Here, however, molecular details are scarce, as there are no structural data available for ATRMec1. This RAD9Ddc1-TopBP1Dpb11 pathway for ATRMec1 activation is also found in budding yeast. Dpb11 is recruited by Ddc1, which is phosphorylated by Mec1 , although either Ddc1 or Dpb11 can activate Mec1 on its own [55,91,92]. The responsible regions of Dpb11 and Ddc1 have been mapped to their unstructured C-terminal tails, within which two conserved hydrophobic residues are important for Mec1 activation [87,92,93].
How Ddc1 and Dpb11 act together to activate Mec1 is still under debate, and it may vary in a cell-cycle dependent manner (Figure 2C). Burgers’ laboratory suggests that whereas the 9-1-1 subunit Ddc1 is responsible for the activation of Mec1 in response to DNA damage in G1 phase, 9-1-1 and Dpb11 cooperate to activate Mec1 in G2/M phase [92,94]. Dpb11 interacts with phosphorylated Rad9, which is modified by a cell-cycle regulated Cyclin-dependent kinase (CDK). Since CDK is not active in G1, this could explain why Dpb11 function is cell-cycle specific . In contrast, Puddu et al. have shown that Dpb11 and 9-1-1 act together in G1, while 9-1-1 is the predominant Mec1 activator in G2 . Fission yeast Rad4/Cut5Dpb11 similarly assists Rad3Mec1 activation in G1, when DSB resection is restricted . This observation suggests that Rad4/Cut5Dpb11 compensates for limited ssDNA to promote full Rad3Mec1 activation in G1 phase .
In S-phase cells, several proteins have been reported to activate Mec1, apparently in a redundant manner. The 9-1-1 complex is recruited to stalled replication forks, and facilitates Rad53 phosphorylation [27,97]. However, dpb11 or ddc1 mutations that interfere with Mec1 activation (or a mutation defective in 9-1-1 loading such as rad24Δ), alone or in combination, show only mild defects in Rad53 phosphorylation in response to replication stress [87,92,98]. Recently, Burgers’ laboratory has reported that Dna2, a conserved nuclease-helicase that is essential for Okazaki fragment maturation, has a role in Mec1 activation in S phase. TEL1 and DDC1 deletions (which also compromise Dpb11-mediated Mec1 activation) were combined with a mutation in the Mec1 activation domain of Dna2, and this eliminated Rad53 activation upon HU treatment in S phase . These data indicate that Dna2 functions as a third factor contributing to Mec1 activation in S phase. Here it is important to note that Dna2 binds the yeast RecQ helicase Sgs1, and that the two factors co-activate each other . Sgs1 also promotes replication stress-dependent checkpoint activation, and the checkpoint defects of a SGS1 deletion are strongly aggravated by mutations in 9-1-1 or the RAD24 gene [97,101,102]. Sgs1 directly binds Rad53 in a Mec1-dependent manner, arguing that it does not simply generate structures that activate and require Mec1 .
Whereas Ddc1, Dpb11 and Dna2 were all shown to enhance Mec1 catalytic activity in vitro, the molecular details of how they act on Mec1 are unclear. Rather than stimulating Mec1 through its kinase domain, they may serve as scaffolds that bring factors closely together. Indeed, Berens and Toczyski have shown that an artificial co-localization of Ddc2 and the replication checkpoint mediator Mrc1 elicits a downstream Rad53 kinase response in the absence of Dpb11 and Ddc1 . It remains possible the Dna2 serves as the crucial activator in this case, and it would be interesting to see how a dna2 mutant defective for Mec1 activation, or the dna2 dpb11 and dna2 ddc1 double mutants, would behave in the Toczyski assay.
Another level of ATRMec1 regulation may be inherent to the kinase itself. Recently it has been reported that human ATRMec1 can autophosphorylate in trans, and that this phosphorylation correlates with ATRMec1 activation [104,105]. It has been suggested that ATRMec1 autophosphorylation assists its binding to TopBP1Dpb11, which further activates the kinase  (Figure 2B). However, another study concluded that mutation of the same autophosphorylation site does not have a strong impact on ATRMec1 function , and the relevant target residue, Thr 1989, is not conserved in budding and fission yeast . Indeed, a study that mutagenized all [S/T]Q sites in checkpoint proteins in fission yeast did not find a single [S/T]Q residue in Rad3Mec1 critical for its function . However, since the ATRMec1 autophosphorylation site does not match the [S/T]Q consensus [104,105], these results are inconclusive. It remains to be seen whether a similar autophosphorylation mechanism exists for Rad3Mec1 in fission yeast or for Mec1 in budding yeast.
ATRMec1 may be also controlled by regulated protein complex formation. It has recently been determined that Nek1 (Never in mitosis A-related kinase 1) promotes ATRMec1-ATRIPDdc2 association in a DNA damage independent manner. The responsible phosphorylation site is unknown, but Nek1 does not seem to target ATRMec1 Thr 1989 directly . Functional complexes may require disruption of dimers. ATMTel1 forms inactive dimers, which dissociate upon autophosphorylation after DSB induction . ATRMec1-ATRIPDdc2 can also form oligomers [109,110,111,112,113] and it has been speculated that the oligomerization of ATRMec1-ATRIPDdc2 regulates kinase activity, even though oligomerization is independent of DNA damage or replication stress [110,112]. There are conflicting reports on the size of ATRMec1-ATRIPDdc2 complexes, ranging from 300 to 1,000 kD in size [86,110,114]. In humans, both the coiled-coil domain of ATRIPDdc2 and ATRMec1 contribute to oligomerization  (Figure 2A). Mutation of the human ATRIPDdc2 coiled-coil domain does not impair chromatin binding, but impairs foci formation and signaling. Interestingly, this coiled-coil mutation shows stronger defects in the replication checkpoint than in the G2/M damage checkpoint [109,110]. In contrast, it has been reported that in Xenopus the ATRIPDdc2 coiled-coil domain is dispensable for both oligomerization and CHK1Chk1 phosphorylation , indicating species or cell-type specific differences.
3. Activation of Effector Kinases
Once ATRMec1 and ATMTel1 have been activated, these kinases signal to the downstream effector kinases, Rad53 and Chk1 (Figure 1). Although Rad53 is more closely related to CHK2Rad53 by sequence, its function is taken over by CHK1Chk1 in higher organisms. In response to stalled replication forks, the signaling of the replication checkpoint is mediated primarily through Rad53 in budding yeast, or by its functional homolog CHK1Chk1 in mammalian cells. Rad53 contains a kinase domain which is flanked by two Forkhead associated (FHA) domains, that can bind phosphorylated proteins . Mutations of critical residues in the FHA domains have revealed that full Rad53 activation requires at least one functional FHA domain, and that mutations of FHA2 show slightly stronger defects [16,116,117]. N-terminal of each FHA domain is an [S/T]Q cluster domain , which becomes modified at multiple residues by either Mec1 or Tel1 [23,24,119]. Nonetheless, genetic evidence indicates that either Mec1 or Tel1 is required, but not sufficient, for Rad53 activation [73,120]. Rad53 activation is facilitated by at least two mediator proteins: the budding yeast Rad9 fulfills this role in the DNA damage checkpoint, while Mrc1 serves as mediator during replication checkpoint activation [16,25,120,121]. Interestingly, Rad53 also appears to be phosphorylated in a cell-cycle dependent manner, and this phosphorylation may fine-tune the checkpoint response . Here we will first summarize the well-characterized molecular mechanism of Rad53 activation by Rad9, and then review current knowledge about Mrc1.
3.1. Rad53 Activation Is Mediated by Rad9 in Response to DNA Damage
Rad9 was the first cell-cycle checkpoint protein identified in budding yeast, and it has a key role as an adaptor for activating Rad53 in the DNA damage response, yet it has little or no role in replication checkpoint triggered by HU-arrested forks [2,123]. Rad9 does not possess enzymatic activity, but contains both tandem Tudor and BRCA1 C-terminal (BRCT) domains. Thus, the mammalian proteins BRCA1, MDC1 and 53BP1 are all considered to be functional homologs of Rad9. The Rad9 tandem Tudor domains can bind to histone H3 methylated on lysine 79 , which is deposited throughout the genome by the methyltransferase Dot1 . In addition, Rad9 binds histone H2A phosphorylated on serine 129, using its tandem BRCT domains . The H2A phosphorylation is mediated by both Tel1 and Mec1 at sites of DNA damage [29,32,127], and both H3K79 methylation and phosphorylated H2A are thought to recruit Rad9 to damaged sites. Consistently, strains bearing dot1Δ or phospho-acceptor mutations in H2A show defects in the G1 checkpoint activation [124,126,128,129]. The G2 checkpoint, on the other hand, still functions in the dot1 mutant. Because the G2 checkpoint activity is lost in a dpb11 dot1 double mutant, it appears that Dpb11 may also recruit Rad9 in G2 (see Section 2.3) [56,93].
Rad9 becomes phosphorylated by Mec1/Tel1 in response to DNA damage, and is required for efficient Rad53 activation . It has been shown that phosphorylated Rad9 can bind to the Rad53 FHA domains, with a preference for FHA2 [16,73,117,130,131]. This, together with the observation that the autophosphorylation of Rad53 is concentration-dependent, has led to the hypothesis that phosphorylated Rad9 locally increases Rad53 concentration, providing a scaffold for efficient Rad53 autophosphorylation and activation [26,132]. In this model, Mec1 would be only required for initial Rad9 phosphorylation, and might not necessarily act directly on Rad53. However, more recent studies have shown that direct phosphorylation of Rad53 by Mec1/Tel1, and not only of Rad9, is required for Rad53 activation [16,132]. Indeed, the mutation of Mec1/Tel1 target sites in Rad53’s N-terminal [S/T]Q cluster domain reduced viability, replication and damage checkpoint functions, as well as its kinase activity .
Activation of the mammalian Rad53 homolog CHK2Rad53 or S. pombe Cds1Rad53 requires phosphorylation of one specific residue by upstream kinases (threonine 68 or 11, respectively) [133,134]. In the case of Rad53, the [S/T]Q sites in the N-terminal SCD seem to be redundant, and phosphorylation at multiple sites is important for activation [16,118]. Once Rad53 has been primed by Mec1/Tel1 and fully activated through autophosphorylation, Rad9 seems to release Rad53, enabling the transduction of the checkpoint responses throughout the nucleus . Mec1/Tel1 phosphorylation of Rad9 also leads to oligomerization of Rad9. The oligomerization may be dispensable for Rad53 activation, whereas it is needed for maintenance of the checkpoint . Intriguingly, phosphorylation of Rad9 by Rad53 disrupts Rad9 oligomerization, providing a negative feedback mechanism for checkpoint regulation .
3.2. Mrc1 Serves as a Mediator in the Replication Checkpoint
Whereas Rad9 acts in response to DNA damage in G1 and G2, Mrc1 is a key mediator protein for Rad53 activation in the context of DNA replication [25,136,137]. Mrc1 is a component of the replisome, and it travels along with replication forks [27,136]. Mrc1 enhances Rad53 activation during replication stress, but does not activate Mec1 kinase activity per se. Rather, it seems to positively influence the enzyme-substrate interaction between Mec1 and Rad53, and could, therefore, recruit Rad53 to stalled forks to facilitate Rad53-Mec1 interaction . Analogously, Claspin, the Mrc1 homolog in higher eukaryotes, contributes to replication checkpoint activation by interacting with CHK1Chk1, a functional homolog of Rad53, and facilitating its activation [139,140,141].
In addition to its checkpoint function, Mrc1 appears to have a structural role in replication fork maintenance, as it binds to the replisome via the Csm3-Tof1 fork protection complex . Mrc1 interacts with Pol ε and Mcm6 [143,144], and Tof1 and Csm3 interact with Mcm2 . Thus, it has been suggested that this complex forms a bridge between the leading strand polymerase ε and the replicative helicase . Consistently, replisome structure is aberrant in the mrc1Δ mutant; mrc1Δ cells proceed faster through S phase, show an uncoupling of the replisome from the site of DNA synthesis and have impaired recovery from HU arrest [25,27,145,146]. Mrc1 becomes phosphorylated by Mec1 and a mutant in which all Mrc1 [S/T]Q sites are mutated to AQ, shows a defect only in checkpoint signaling, but not in replisome progression [25,136]. By using this mrc1-AQ mutant, an alternative function for Mrc1 in the replication checkpoint has been suggested, namely, phosphorylation of Mrc1 may stabilize the association of Mec1 with sites of stalled replication forks, thereby creating a positive feedback for Mec1 function . Although Mrc1 activates the replication checkpoint in response to replication stress, loss of it can be compensated by Rad9 . Indeed, Rad9 accumulates at stalled replication forks in mrc1Δ cells on HU . It is therefore likely that loss of Mrc1 checkpoint function creates DSBs or damage structures that provoke Rad9-dependent Rad53 activation. This is a much more likely option than that Mrc1 and Rad9 are equivalent in their mode of action, given that Mrc1 is part of the replisome, and Rad9 clearly is not.
3.3. A Role for Sgs1 in Rad53 Activation
Another factor that has been shown to be involved in replication checkpoint signaling and which helps activate Rad53, is the budding yeast RecQ helicase, Sgs1 [97,103]. Sgs1 interacts with Dna2, RPA and Rad53, and is constitutively associated with replication forks [100,103,148]. The deletion of sgs1 alone destabilizes DNA polymerases α and ε when replication forks are stalled by HU , and this effect is far more pronounced when combined with either mrc1Δ or the S-phase specific mutant allele of MEC1, mec1-100 [32,112,148,149,150]. This leads to a synergistic arrest of growth and failed fork recovery in response to HU, and a loss of dNTPs incorporation, as both polymerases α and ε are lost from the replisome . One explanation of the observed synergy may be that mec1-100 generates fold back structures that need Sgs1 for resolution. However, Sgs1 also participates in the activation of Rad53, particularly in response to HU arrest . Sgs1 itself contains a [S/TQ] cluster that is phosphorylated in a Mec1-dependent manner in vivo and in vitro . When phosphorylated, this domain of Sgs1 binds Rad53, again both in vivo and in vitro [97,103]. Therefore, Sgs1 serves as a replication checkpoint mediator that recruits Rad53 to stalled forks, acting in much the same way as Rad9 acts at DSBs. Mrc1 and Sgs1 have been found to be epistatic for their function in Rad53 phosphorylation, although Sgs1 functions in parallel with Rad24 and 9-1-1, and the double mutants are highly compromised for the activation of Rad53 at stalled forked [97,102]. A RecQ homologue in mammals, the WRNSgs1 helicase, has also been shown to facilitate the ATRMec1-CHK1Chk1 checkpoint pathway in response to camptothecin , indicating that RecQ function in checkpoint signaling may be conserved.
4. Targets of the Replication Checkpoint
An activated replication checkpoint cascade transduces a multitude of stimuli that control cell-cycle and replication-fork recovery. Here, we summarize the downstream pathways that are influenced by the checkpoint. We focus on relevant replication fork targets at the end of this section, to the extent that they are known.
4.1. Cell Cycle Regulation
In all eukaryotic species, checkpoint effector kinases play a central role in cell-cycle arrest upon checkpoint activation, even though the mechanism of arrest differs. In fission yeasts and higher eukaryotes, CHK1Chk1 and CHK2Rad53 negatively regulate CDC25Mih1 phosphatases that remove inhibitory phosphorylation on cyclin-dependent kinase (CDK) [152,153,154,155]. Checkpoint-dependent phosphorylation of CDC25Mih1 down-regulates its activity through inhibition of its nuclear localization by binding 14-3-3, a nuclear-cytoplasmic shuttling protein, and through degradation by the SCFΒTrcp ubiquitin ligase [156,157,158]. Therefore, DNA replication and the damage checkpoint down-regulates the CDK cell cycle engine, thereby blocking G2/M transition. In S. cerevisiae, however, this cell-cycle arrest by CDK inhibition does not occur . Nonetheless, checkpoint mutants exhibit a cytologically typical mitotic arrest defect, with elongated spindles in response to blocked replication forks [2,4,160]. Budding yeast cells transmit the checkpoint signal to inhibit progression of mitosis by stabilizing securinPds1, which inhibits the metaphase to anaphase transition, and by stimulating the Bub2/Bfa1 GAP complex which inhibits the mitotic exit network [22,161,162]. Microtubule elongation is also blocked .
4.2. Essential Function for Cell Viability—dNTP Pool Regulation and Fork Maintenance?
Intriguingly, Mec1 and Rad53 and their functional homologs ATRMec1 and CHK1Chk1 are essential for cell proliferation, but this is not due to their checkpoint function [164,165,166,167,168,169]. In S. cerevisiae, the lethality of mec1 or rad53 deletions can be bypassed by up-regulating the dNTP pool with another mutation [164,165]. In S. pombe, on the other hand, neither Rad3Mec1 nor Cds1Rad53 are encoded by essential genes, raising the question of whether the critical dNTP regulating function of these checkpoint kinases is conserved. In mammalian cells NTP pool control through c-Myc appears to be a determinant of dNTP levels .
In S. cerevisiae dNTP levels increase 8-fold in response to DNA damage, an increase that facilitates cell survival, even as it increases the mutation rate . Given the pleotropic effects of dNTPs, it is reasonable that cells have multiple pathways that regulate dNTP concentrations, one of which is through the Dun1 kinase, a target of Rad53 . Dun1 phosphorylates Sml1 and Dif1, which inhibit ribonucleotide reductase (RNR), priming them for degradation [173,174]. Dun1 also induces RNR gene transcription by inhibiting the transcriptional repressor Crt1 . Given that the failure to regulate dNTPs is lethal, these pathways are obviously very important in budding yeast.
A recent study from the Longhese laboratory has revealed that the essential functions of Mec1 and Rad53 can also be bypassed by lowering the activity of Cdc28, the budding yeast CDK . Similarly, a delayed entry into S or M phase provoked by lowered levels of G1- or M-phase cyclins, improves the survival of mec1Δ or rad53Δ cells on low doses of HU. This suggests that either extending G1, prior to S phase entry, or a reduction in the number of active replication forks, compensates for the lethal effects of checkpoint kinase ablation. Most likely, this suppression is explained by the fact that cells have sufficient time both to generate dNTPs and to complete replication. Surprisingly, lowered rates of microtubule elongation provoked by cin8 mutation, also suppresses the lethality of mec1Δ or rad53Δ cells. Both survival on HU and the ensuing completion of replication improve in the cin8 mutant, suggesting that centromere segregation by a premature mitotic spindle, is another lethal consequence of mec1 or rad53 ablation. Similarly, the inhibition of microtubule elongation through nocodazole diminishes Rad52 repair foci, which are induced in cells bearing the temperature-sensitive mec1-14 mutation at elevated temperatures in S phase . Together, these data suggest that checkpoint kinases also coordinate the completion of replication with microtubule elongation, in line with the original concept of checkpoints: that is, to preserve the order of cell-cycle events. We note, however, that the experiments in the cited study used either no or low dose HU. Indeed, after treatment with high doses of HU or MMS, the essential function of the replication checkpoint was shown to be its ability to facilitate the restart of replication forks once the lesions have been removed [34,164]. This may act by preventing the accumulation of aberrant DNA structures and/or fork collapse [32,148,149,150] (see Section 4.6).
Mec1 and ATRMec1 are known to prevent chromosome breakage at fragile sites where replication forks frequently slow down, even in the absence of exogenous damage [31,177]. Given the fact that ATRMec1 is an essential protein in mammalian cells, and given that there are many more obstacles that impair fork progression in higher eukaryotes, it may well be that overcoming intrinsic replication stress is the essential role for ATRMec1/CHK1Chk1 in higher organisms.
4.3. Replication Origin Control
DNA replication is initiated by a series of steps that proceed in a sequential manner. In the first step, known as licensing, the pre-replicative complex (pre-RC) is loaded onto DNA at the origins of replication in G1 phase, when CDK activity is low. The pre-RC consists of ORC, Cdc6, Cdt1, and Mcm2-7. In the second step, the essential helicase components Cdc45 and GINS, together with DNA polymerases, are brought onto pre-RC by the bridging factors Sld3-Sld7 and Dpb11-Sld2 in a CDK- and DDK- (Dbf4 dependent kinase, CDC7Cdc7-DBF4Dbf4) dependent manner [178,179]. Finally, Mcm10 functions in the unwinding step, together with the CMG helicase complex (Cdc45-MCM-GINS), thus initiating DNA replication [180,181,182].
In eukaryotes, there are multiple origins of DNA replication (in S. cerevisiae ~500 in a haploid genome), and those initiation events are regulated temporally [183,184]. In budding yeast cells, several factors essential for the initiation of DNA replication are limiting, and those factors appear to be recycled for later initiation events [185,186]. Recent studies have shown that Rad53 targets and inactivates two of the limiting replication factors, Sld2 and Dbf4 (the DDK regulatory subunit). Thus, late-origin firing is suppressed by an activated replication checkpoint [187,188]. Regulation of replication initiation by the checkpoint also occurs in higher eukaryotic cells, although the targets appear to be different . It is interesting to note that in S. pombe and mammalian cells, the DDK has been shown to have a positive role in replication checkpoint activation [190,191,192]. DDK also modulates the checkpoint response to facilitate DNA repair and the recovery from checkpoint arrest [193,194] (see Section 4.5).
4.4. Transcription Control
Genome-wide gene expression analyses in budding yeast have revealed that hundreds of genes are up- or down-regulated upon treatment with genotoxic reagents that induce stalled replication forks [127,195,196,197]. This transcriptional regulation is controlled by two branches in the replication checkpoint pathway; one directly by Rad53, the other by Dun1 [175,198,199]. Dun1 phosphorylates and inhibits Crt1, which recruits repressors Ssn6 and Tup1 to the promoters of DNA damage response genes. Dun1 thereby up-regulates genes involved in DNA repair and ribonucleotide biosynthesis [175,196]. Two recent studies have revealed that the cell-cycle dependent genes that are transcribed at the G1/S boundary are also induced as a part of the DNA replication and damage response in S. cerevisiae [198,199]. Over 200 G1/S genes are regulated by the heteromeric transcription factors SBF (Swi4-Swi6 cell-cycle box (SCB) binding factor) and MBF (MluI cell-cycle box (MCB) binding factor) . While SBF activates transcription in G1, MBF down-regulates transcription outside of G1 through the co-repressor Nrm1, thereby restricting the expression of the target genes in late G1 [201,202]. The studies revealed that MBF target genes are up-regulated upon replication stress by inactivation of Nrm1 in a Rad53-dependent Dun1-independent manner [198,199]. This transcriptional regulation is also conserved in S. pombe, as the Rad53 homolog Cds1Rad53 inhibits Nrm1 and promotes G1/S transcription in response to replication stress [202,203].
4.5. Coordinating DNA Repair
It seems obvious that the DNA damage checkpoint should be coupled with the up-regulation of DNA repair, and various forms of damage provoke both a checkpoint response and DNA repair. Since ssDNA coated by RPA initiates both checkpoint activation and the loading of Rad51 for repair by HR, the cell has to carefully coordinate these events, particularly at the replication fork where ssDNA exists constitutively. Importantly, studies in budding and fission yeasts have shown that the replication checkpoint actively suppresses the initiation and processing required for HR [204,205,206]. Rad52 foci are absent in cells treated with HU, even in the presence of DSBs, as long as the replication checkpoint is intact [204,205]. Consistently, the ATRMec1-p53 pathway has been shown to suppress the formation of RAD51Rad51 foci in response to HU in mammalian cells , although in other cases it has been reported that CHK1Chk1 phosphorylates RAD51Rad51 and positively regulates HR in response to HU or CPT [208,209]. These discrepancies suggest that the checkpoint regulation of HR is fine-tuned with respect to the type and level of damage.
In addition to the role of 9-1-1 in ATRMec1 activation described above, it has been well documented that 9-1-1 functions in various aspects of DNA repair (see review ). Indeed, cells may use the multi-tasking capacity of 9-1-1 to coordinate the checkpoint activation with DNA repair. In both S. cerevisiae and S. pombe, it has been suggested that 9-1-1 functions in DNA damage tolerance pathways [211,212,213]. The budding yeast 9-1-1 complex also contributes to the resection of DSBs, as rad24 mutants that impair 9-1-1 loading have reduced ssDNA formation and impaired recruitment of Mec1 at HO-induced breaks [48,214]. Finally, human 9-1-1 physically interacts with factors involved in base excision repair (BER), such as MYH, Polβ, TDG, Fen1, and DNA ligase I, and stimulates their enzymatic activities [215,216,217,218,219,220].
How then is 9-1-1 function regulated? Recent studies have indicated that post-transcription modification of 9-1-1 is crucial for its regulation. S. pombe Rad9Ddc1, a component of checkpoint clamp, is phosphorylated at multiple sites by Rad3Mec1, and two phosphoacceptor sites in the C-terminal tail that promote Rad9Ddc1-Cut5Dpb11 interaction are required for Chk1Chk1 activation  (see Section 2.1). Interestingly, Rad3Mec1-dependent phosphorylation at T225 on Rad9Ddc1 has been shown to facilitate the interaction with Mms2Mms2-Ubc13Ubc13, a ubiquitin-conjugating enzyme, to promote error-free repair . Finally, a study in human cells has indicated that RAD18Rad18 facilitates RAD9Ddc1 recruitment at IR-induced damage through an unknown mechanism, although this mode of RAD9Ddc1 recruitment has little impact on checkpoint activation: no loss of CHK1Chk1 or CHK2Rad53 activation was scored in cells depleted for RAD18Rad18 .
As mentioned above, Dbf4-Cdc7 also modulates checkpoint activity [190,191,192,194]. A recent study by Furuya et al. has shown that the S. pombe DDK phosphorylates Rad9Ddc1 , thereby reducing interaction between Rad9Ddc1 and RPA and releasing 9-1-1 from chromatin. This phosphorylation appears to be important for the repair of CPT-induced lesions, as the number of Rad22Rad52 repair foci increase in DDK phospho-acceptor site mutants . Together, the above studies argue compelling that 9-1-1 functions at the interface of repair and checkpoint pathways.
4.6. Replication Fork Stability
As briefly discussed in Section 4.2, maintenance of replication fork integrity is the crucial function of the replication checkpoint in response to replication stress (Figure 3). Control of the cell cycle, transcription, and origin firing, while important, are non-essential events based on the following considerations: (1) Blocking the transition through M phase by nocodazole is not sufficient to rescue the lethality of high doses of MMS or HU in rad53 or mec1 mutant cells [12,164]; (2) De novo protein synthesis does not contribute to cell viability, nor is it required for the resumption of replication forks after HU treatment ; and (3) HU treatment of cells that cannot suppress late-origin firing due to phospho-site mutations in both Dbf4 and Sld3, is not lethal [187,188]. On the other hand, checkpoint mutants fail to resume DNA replication after transient exposure to HU or MMS, and replisome components are not detected by chromatin immunoprecipitation (ChIP) at early origins on HU in mec1 mutants [32,34,148,150]. Finally, upon fork stalling, checkpoint mutants show aberrant DNA structures, such as the formation of reversed forks (so-called chicken-foot structures) and an accumulation of ssDNA [33,149]. These observations led to the notion that the replication checkpoint maintains the stable association of replication polymerases at stalled forks, and prevents the formation of pathological fold-back structures in face of replication stress.
An important aspect of the replication checkpoint is the fact that the roles of ATRMec1 and the effector kinase, CHK1Rad53, are not equivalent, particularly with respect to fork recovery. This is well-documented, yet often overlooked. In S. cerevisiae it has been shown that the function of Rad53 that is essential for viability on MMS is largely rescued by deletion of the exonuclease, EXO1 . This suggests that the down-regulation of Exo1 at arrested forks is a major function of Rad53 . Interestingly, however, an EXO1 deletion does not suppress mec1Δ lethality, indicating that Mec1 has functions that are crucial for cell survival on MMS, other than the activation of Rad53 . Indeed, when forks are arrested by high concentrations of HU, quantitative ChIP assays showed that leading and lagging DNA polymerases are displaced in mec1 mutant cells, although their association is intact in the absence of Rad53 [32,148,150]. Similarly, in mec1 mutants Cdc45 becomes undetectable at early origins [27,32]. In rad53 mutants, on the other hand, ChIP signals for DNA polymerases stay high, yet the distribution of the MCM helicase is altered, underscoring again the distinct roles played by Mec1 and Rad53 at stalled forks [32,148]. The exact mechanisms are unclear, yet it appears that Mec1 activity keeps replication polymerases engaged in the presence of HU, while Rad53 acts primarily through the MCM helicase to ensure replication restart [32,148].
Whereas the outcome of checkpoint activation is clear, it is not clear exactly what happens to the stalled replisome complex in the absence of a functional replication checkpoint. ChIP data led to the conclusion that the replication checkpoint stabilizes the replisome and preserves its integrity (Figure 3C). Recent biochemical approaches, on the other hand, have shown that intact replisome complexes can be recovered by Sld5-(GINS) pull-down even in mec1 and rad53 checkpoint mutants arrested with HU. The replisome complex, even though it is not functionally engaged, may stay chromatin-associated in both checkpoint-proficient and -deficient cells . ChIP coupled with deep sequencing suggests that the replisome moves away from the last site of DNA synthesis in an uncoordinated manner in checkpoint mutants, possibly still unwinding double-stranded DNA by the helicase, but not incorporating dNTPs (Figure 3D). This random sliding of the replisome could explain the apparent “loss” of polymerases from early initiating sites, as well as the accumulation of ssDNA observed by ChIP and electron microscopy analyses [27,32,33,150,224]. This movement, however, was only detected at the very earliest origins , did not correlate with the incorporation of nucleotides [27,225], and could not account for the majority of forks where both polymerase and helicase seem to persist even in checkpoint mutants . One model that could reconcile these results suggests that the replication checkpoint keeps the replisome engaged at sites of stalled forks in an as yet undefined way, rather than simply tethering the replisome factors together. Further work is needed to address the molecular mechanisms of how the replication checkpoint facilitates the restart of stalled forks and recovery from replication stress.
Several studies have indicated that the checkpoint regulates the action of nucleases and helicases at stalled forks (Figure 3E,F). Indeed, in S. cerevisiae the exonuclease Exo1 is modified and inhibited by the DNA damage checkpoint [223,226], and deletion of EXO1 has been shown to suppress the accumulation of ssDNA in checkpoint-deficient cells on HU . Similarly, human EXO1 undergoes ubiquitin-mediated degradation on HU, in a manner partly dependent on phosphorylation by ATRMec1 . Recently it has also been shown that the nuclease/helicase Dna2 is a target of the replication checkpoint, and that phosphorylation facilitates its association to DNA following exposure to HU . Dna2 nuclease activity is thought to prevent ssDNA formation by cleaving off ssDNA tails that arise from stalled fork regression . A structure-specific nuclease Mus81-Eme1 is also a target of Cds1Rad53 in S. pombe, and is released from chromatin upon fork stalling . Finally, as mentioned above, the Sgs1 helicase is also a target of Mec1, and its helicase activity has an important role in replication fork stability [32,101,103,148]. Specifically, when sgs1Δ is combined with either mrc1Δ or mec1-100, an S phase-specific mec1 mutant , then total fork collapse occurs in the presence of HU, and neither DNA polymerases nor RPA can be detected at the collapsed fork by ChIP analysis [32,101,103,148]. This results in a dramatic increase in gross chromosomal rearrangements and high levels of cell lethality. The mammalian RecQ helicases BLMSgs1 and WRNSgs1 are also phosphorylated by ATRMec1 and ATMTel1 [231,232], and are also implicated in replication fork recovery after stress [231,233,234]. Whereas Sgs1 helicase does help transduce the checkpoint signal by activating Rad53, the prevention of fork collapse requires its helicase activity, and not simply its ability to bind Rad53, but rather depends on the Sgs1 helicase activity [32,101,103,148]. Together, these data suggest that ATRMec1 and CHK1Rad53 target distinct sets of nucleases and helicases to suppress the formation of pathological fork structures that impair replication fork resumption after removal of the damage.
A number of studies has been carried out to identify the targets of the replication and DNA damage checkpoints, and to determine their relevance for the checkpoint response. In Table 3, we list the replication fork associated factors in addition to those mentioned above, that are phosphorylated upon checkpoint activation [235,236,237,238,239,240,241]. While phosphoproteomic studies have identified many, only a few checkpoint targets have been elucidated in depth. For example, RPA1Rfa1, the large subunit of RPA, is crucial for ATRMec1 recruitment at the site of damage and is a documented Mec1 target , yet the function of its phosphorylation remains unclear. In human cells, the second RPA subunit, RPA32Rfa2, is phosphorylated by ATRMec1 and by another PIKK, DNA-PK, upon replication stress, and this phosphorylation is required for a robust checkpoint response and for recovery from fork arrest . DNA polymerase α, the lagging strand polymerase, is a checkpoint target , and has an important role in the initiation of the replication checkpoint . Interestingly, the gap-filling lagging strand polymerase, DNA polymerase δ, has been shown to be a target of Mec1 in response to MMS , although the importance of its phosphorylation in replication fork stability is unclear. The active replicative helicase CMG complex (Cdc45-MCM-GINS) is one of the common targets of the replication and damage checkpoint in different organisms; MCM2Mcm2 (primarily a target of ATRMec1 [245,246,247]), MCM3Mcm3 (primarily a target of ATMTel1-CHK2Rad53 [245,248,249]), MCM4Mcm4 (a target of both ATRMec1 and ATMTel1 pathways [249,250,251]), GINS component Psf1 (a Mec1 target ), and PSF2Psf2 (a CHK2Rad53 target ) are all phosphorylated upon checkpoint activation. CHK2Rad53 phosphorylation of the CMG inhibits its helicase activity , whereas phosphorylation of Mcm2 has been shown to recruit Plk1Cdc5 (polo-like kinase 1) on chromatin and promote checkpoint recovery  (see also Section 5). Finally, analyses in both budding yeast and human cells have identified specific phosphopeptides of the GINS complex and DNA polymerase ε targeted by ATRMec1 and/or ATMTel1, and not by downstream effector kinases CHK1Rad53 (see Table 2 [236,237]), consistent with the distinct roles played by ATRMec1 and the downstream effector kinase CHK1Rad53. It is of utmost relevance to find out whether, and how, phosphorylation of these factors impacts replication fork integrity and fork restart.
|Table 3. Replication factors modified by replication and damage checkpoint kinases.|
|S. cerevisiae (targeted by)|
|Rfa1 (Mec1/Tel1)||a subunit of RPA||[235,236,252]|
|Rfa2 (Mec1/Tel1)||a subunit of RPA||[236,242]|
|Pol1 (Rad53)||a subunit of DNA polα|||
|Pol12 (Rad53)||a subunit of DNA polα|||
|Pol31 (Mec1/Tel1)||a subunit of DNA polδ|||
|Dpb4 (Mec1/Tel1)||a subunit of DNA polε and ISW2||[235,236]|
|Mcm4(Mec1)||a subunit of MCM|||
|Mcm6 (Mec1)||a subunit of MCM|||
|Psf1 (Mec1)||a subunit of GINS|||
|Mrc1 (Mec1, Rad53)||checkpoint mediator||[25,236]|
|Tof1 (Rad53)||fork protection complex|||
|Ctf4 (Rad53)||polα interactor|||
|Dbf4 (Rad53)||a subunit of DDK||[187,188,236,253]|
|Sgs1 (Mec1)||RecQ helicase|||
|RPA1 (ATR/ATM)||a subunit of RPA||[237,241]|
|POLE (ATR/ATM)||a subunit of polε|||
|POLEE4 (ATR/ATM)||a subunit of polε|||
|POLL (ATR/ATM)||a subunit of polλ|||
|MCM2 (ATR/ATM)||a subunit of MCM||[237,239,241]|
|MCM3 (ATR/ATM)||a subunit of MCM|||
|MCM4 (ATR/ATM, CHK1)||a subunit of MCM||[237,239,250]|
|MCM5 (CHK1)||a subunit of MCM|||
|MCM6 (ATR/ATM)||a subunit of MCM||[237,239]|
|MCM7 (ATR/ATM)||a subunit of MCM|||
|POLDIP3 (ATR/ATM)||Polδ interacting factor|||
|MCM10 (ATR/ATM)||replication initiation factor|||
|HELB (ATR/ATM)||DNA helicase B|||
|RFC1 (ATR/ATM)||clamp loader||[237,239,241]|
|RFC3 (ATR/ATM)||clamp loader|||
|PSF2 (ATR/ATM)||a subunit of GINS||[237,241,249]|
|ORC3 (ATR/ATM)||a subunit of ORC|||
|ORC6 (ATR/ATM)||a subunit of ORC|||
|DBF4 (ATR/ATM)||a subunit of DDK|||
|Claspin (ATR/ATM)||checkpoint mediator||[237,241]|
|CAF-1B (ATR/ATM)||histone assembly|||
|CTF18 (ATR/ATM)||POLH interactor||[237,241]|
|TopBP1 (ATR/ATM, CHK1)||initiation and ATR activation||[237,240,241]|
|WDHD1 (ATR/ATM)||Polα interactor|||
|BLM (ATR/CHK1)||RecQ helicase||[241,240]|
|FEN1 (CHK1)||5' flap endonuclease|||
|DNA Ligase 1 (CHK1)||DNA ligase||[239,240]|
|TIPIN (ATR/ATM)||fork protection complex|||
|WRN (ATR/ATM)||RecQ helicase||[232,237]|
In addition to direct fork-associated proteins, enzymes that modulate long-range chromatin organization are also shown to be targets of the replication checkpoint. For instance, the Ino80 and Isw2 chromatin remodelers are confirmed targets of the DNA damage checkpoint [236,254]. These ATP-dependent chromatin remodeling complexes are shown to be recruited at the stalled replication forks and to promote the recovery of stalled forks [255,256,257,258]. Loss of Ino80 chromatin remodeling activity results in a poor resumption of stalled forks and an increase in DNA repair response [255,256,258]. This correlates with a proposed action of removing nucleosomes to allow fork progression, although INO80 contains a 5' to 3' DNA helicase activity in its Rvb1 and Rvb2 subunits, which may also be involved in altering fork structure . Another study has suggested a role in checkpoint down-regulation, although how chromatin remodeling reduces a checkpoint response is unknown ( see also Section 5.2).
A recent study has proposed that the replication checkpoint releases topological tension generated by a transcribed gene that is tethered to components of the nuclear pore . DNA replication forks frequently pause at transcribed genes, and this pausing is independent of the directionality between replication and transcription . Bermejo et al.  have determined that mutations in THO, TREX-2, or inner basket nucleoporins enhance the survival of rad53 mutants on HU, and rescue fork reversal, which occurs in the checkpoint mutants on HU . The replication checkpoint appears to target Mlp1, a nucleoporin, and counteracts its function for gene tethering to the nuclear pore. A phospho-mimicking mlp1 mutation suppresses rad53Δ lethality on HU, suggesting that releasing topological impediments generated by gene gating is one task of the replication checkpoint. However, the interpretation may be rather complicated since loss of Mlp1 also releases and alters the activity of the SUMO-protease Ulp1 which plays a key role in DNA repair .
A bioinformatics approach has also indicated that ATRMec1 and ATMTel1 may be involved in regulating a broad range of cellular structures, such as the spindle pole body/centrosome and actin cytoskeleton . It will be interesting to explore whether those less canonical Mec1/Tel1 targets function at replication forks to ensure genome stability.
5. Checkpoint Recovery
5.1. Protein Phosphatases Down-Regulate the Checkpoint
The kinase cascade of DNA replication and damage checkpoints must be down-regulated to continue the cell cycle once the impediment is removed (Figure 4). This process is known as recovery, and correlates with the disappearance of hyper-phosphorylated Rad53 in S. cerevisiae. Recovery is distinct from “adaptation”, which refers to the down-regulation of the checkpoint despite the persistence of unrepaired DNA damage (see review ). A reasonable way to counteract the kinase cascade would be through protein phosphatases (see review ). In S. cerevisiae, PP2C phosphatases Ptc2 and Ptc3, and PP4 protein phosphatase Pph3 have been shown to function in the recovery from HO endonuclease-induced single DSB response [266,267,268]. Rad53 down-regulation requires Pph3 and Ptc2 in response to MMS [269,270] and another PP1 phosphatase, Glc7, in response to HU  (Figure 4A). The dephosphorylation of Rad53 is important for the resumption of stalled replication forks after removal of the drugs [270,271]. It has been suggested that the phosphatases non-redundantly target Rad53 in response to different types of damage (DSBs vs. MMS) . However, the loss of those phosphatases at the same time impairs cell growth, suggesting that they have overlapping functions that support cell viability .
A role for protein phosphatases in checkpoint recovery is conserved in other eukaryotes: PP1 phosphatase Dis2Glc7 negatively regulates Chk1Chk1 in S. pombe  and, in metazoans, various phosphatases have been found to function in checkpoint down-regulation (see reviews [273,274]). ATMTel1 is targeted by PP2A and Wip1 (Wild-type p53-induced phosphatase 1) [275,276], as are CHK1Chk1 and CHK2Rad53 [277,278,279,280,281]. Wip1 activity is cell-cycle regulated by both protein level and phosphorylation, and it has been suggested that Wip1 protein regulation fine-tunes the global DDR response .
5.2. Phosphatase-Independent Mechanisms of Checkpoint Down-Regulation
In addition to the phosphatase-dependent down-regulation, the ubiquitin proteasome pathway also plays a role in checkpoint recovery in higher organisms (Figure 4B). CHK1 has been shown to be targeted by cullin 1 (CUL1) or cullin 4A (CUL4A) dependent proteolysis [283,284]. ClaspinMrc1 is also targeted by SCFΒ-TRCP for degradation [285,286,287], and this effect is counteracted by USP7, a deubiquitylating enzyme, called ubiquitin-specific protease 7 . PLK1Cdc5 (polo-like kinase 1) promotes ClaspinMrc1 degradation by promoting SCFΒ-TRCP-dependent ubiquitination [285,286,287], and this PLK1Cdc5 activity is prompted by Aurora A and Greatwall kinases [289,290]. The role of PLK1Cdc5 in checkpoint down-regulation may be conserved, since the S. cerevisiae PLK1 homolog, Cdc5, is important for adaptation from an irreparable DSB, although the action of Cdc5 appears to be different [122,291,292,293]. PLK1Cdc5 has also been shown to facilitate DNA replication under replication stress. Namely, the Xenopus PLK1Cdc5, Plx1Cdc5, is recruited to chromatin through ATRMec1-dependent phosphorylation of MCM2Mcm2 at serine 92 upon fork stalling, and Plx1Cdc5 recruitment is important for origin firing near stalled forks . In human cells, it has been shown that PLK1Cdc5 phosphorylates ORC2Orc2 on serine 188. This phosphorylation promotes DNA replication under replication stress . In summary, checkpoint recovery is promoted by two opposing enzymatic activities; multiple phosphatases directly quench the checkpoint kinases and the effectors, while polo-like kinase promotes the degradation of checkpoint factors and facilitates the initiation of replication to complete S phase.
Srs2 helicase and Sae2 in S. cerevisiae appear to facilitate checkpoint recovery by eliminating or reducing the source of the sensor kinase activation. Srs2 restrains homologous recombination by dislodging the Rad51 recombination protein from ssDNA [295,296]. Consistently, SRS2 deletion impairs the down-regulation of the checkpoint after repair of HO-induced DSBs [297,298]. A recent study has demonstrated that srs2Δ cells retain Ddc2- and RPA-focus formation and chromatin association, even when the bulk of the DSB repair has been completed . Yeung and Durocher suggest that Srs2 dismantles Rad51, which results in elimination of ssDNA and a suppression of Mec1 signaling. Sae2 (CtIP in vertebrates) is involved in meiotic and mitotic DSB processing, together with the Mre11-Rad50-Xrs2 (NBS1 in vertebrates) complex [300,301,302,303]. MRNMRX recognizes DSBs very rapidly and recruits ATMTel1 kinase. Several studies have shown that sae2 mutants show a defect in checkpoint recovery, whereas overexpression of SAE2 counteracts checkpoint activation [304,305,306]. Deletion of SAE2 results in an increased level of MRX at DSBs, whereas overexpression of SAE2 has the opposite effect, suggesting that Sae2 limits MRX retention at the damage site and thereby limits Tel1 checkpoint signaling [304,305]. This Sae2 function may facilitate switching from Tel1/ATM to Mec1/ATR signaling (see Section 6).
Recent studies in S. cerevisiae have shed light on yet another mechanism of the checkpoint attenuation that involves a scaffold complex called Slx4-Rtt107 (Figure 4C). The Mec1 activator Dpb11 binds Rad9 and up-regulates Rad53 activation (see Section 2.4) , and the Mec1/Tel1 phosphorylation of H2A at Ser129 (H2A-p; γH2AX in mammals) recruits Rad9 to damage sites, also leading to the activation of Rad53 . Intriguingly, the DNA repair scaffold complex Slx4-Rtt107 can also bind Dpb11 in a Mec1-dependent manner , and the C-terminal tandem BRCT domain of Rtt107 interacts with H2A-P . In a recent study, the Smolka group argues that the Slx4-Rtt107 complex negatively regulates Rad53 by competing with Rad9 for both Dpb11 and H2A-p, two positive checkpoint regulators .
Finally, a recent study suggests that both Isw2 and Ino80, confirmed targets of checkpoint kinases, interact with RPA and function to attenuate the checkpoint activity . Molecular mechanisms of the remodeler function in checkpoint recovery are unclear, but a similar competition may exist where Ino80 and Isw2 exclude excess Mec1 from RPA binding.
6. Coordination between ATRMec1 and ATMTel1
While ATRMec1 and CHK1Chk1 serve distinct in the replication checkpoint, it is also clear that ATRMec1 and ATMTel1 do not have identical roles. ATMTel1 initiates the DSB checkpoint response, while ATRMec1 is the first responder to the replication-associated DNA damage. As mentioned above, MRX/MRN recognizes DSBs and recruits ATMTel1 kinase. However, there are also a range of overlapping functions between ATMTel1 and ATRMec1. At DSBs, budding yeast Tel1 phosphorylates Mre11, Xrs2, and Sae2. Sae2 activates MRX nuclease activity, which initiates 5'–3' end resection . Extended ssDNA formation is followed by Exo1, Sgs1-Top3-Rmi1, and Dna2 helicase/nuclease activity, with the aid of Fun30 (SMARCAD1), a recently identified chromatin remodeler [311,312,313,314]. Extended RPA-coated ssDNA leads to the recruitment and subsequent activation of ATRMec1-ATRIPDdc2  (see Figure 1). Recently, Peterson et al. have shown that ATRMec1 in mammals also phosphorylates CtIPSae2, and this phosphorylation stimulates CtIPSae2 chromatin interaction and end-resection . Thus, checkpoint signaling after DSB induction involves both the ATRMec1 and ATMTel1 pathways. A recent biochemical analysis has suggested a mechanism for a consecutive switch from ATMTel1 to ATRMec1 through swapping the recruiter MRNMRX for RPA at the DSB damage site during end-resection . We note that in higher eukaryotes another PIKK, DNA-PK, also participates in the signaling of the DSB damage response .
Although ATRMec1-ATRIPDdc2 is the major regulator of the replication-associated checkpoint, the vulnerable nature of replication forks can generate DSBs through fork collapse (Figure 1), which then might require ATMTel1 activation to protect the break and facilitate fork restart. In a Xenopus replication system, both ATMTel1 and ATRMec1 appear to play an important role in preventing DSB formation during replication . In the absence of ATMTel1 and ATRMec1, DSBs accumulate and DNA polymerase ε is displaced from CPT- or mitomycin C-damaged chromatin. It has also been demonstrated that MRNMRX is redistributed to the restarting forks, in a manner dependent on ATMTel1 and ATRMec1. This appears to be important for preventing persistent DSB formation . Consistently, Doksani et al. have shown that in the absence of Tel1 or MRX function, abnormal DNA intermediates accumulate when the replication fork encounters an induced DSB. They suggest that the MRX-Tel1 pathway prevents the formation of cruciform structures at the fork-DSB junction , a pathway that may depend on crosstalk between ATRMec1 and ATMTel1.
The mechanism of ATR-ATM crosstalk is somewhat lesion specific. For instance, ATRMec1 phosphorylates ATMTel1 at serine 1981, which promotes the dissociation of inactive ATMTel1 dimers into active monomers, in response to HU or UV. This results in CHK2Rad53 activation and a robust G2/M arrest . In contrast, in the presence of IR-induced damage, ATMTel1 auto-phosphorylates this same residue, resulting in self-activation [108,319]. Screens for novel tel1 mutations in budding yeast has shown that there are overlapping functions between Mec1 and Tel1 with respect to stalled forks, and that some tel1 gain-of-function mutants can even compensate for Mec1 functions. These dominant TEL1-hy mutants suppress mec1Δ deficiency in respect to Rad53 activation and cell survival to different extents, in response not only to DSBs but also to UV, MMS, and HU in S phase . Some Tel1-hy proteins have higher catalytic activity than the wild-type Tel1, yet others show equivalent or even lower activity, suggesting that changes in specificity can also result in suppression. In addition, checkpoint restoration by TEL1-hy mutants in response to DSBs requires Xrs2 interaction, suggesting that Tel1-hy recruitment to damage through MRX is still crucial for the suppression phenotype .
In agreement with the above observations, MRX has been shown to have a role in replication checkpoint signaling, in parallel to its well-characterized function in the DSB response [79,321]. Indeed, budding yeast mre11 mutants show reduced Rad53 activation in response to HU . In mammals, as well, MRNMRX has been shown to function upstream of ATRMec1 in response to UV and HU, and to interact with ATRMec1-ATRIPDdc2 through the FHA and BRCT domains of Nbs1Xrs2 . These data would suggest that MRNMRX functions as a regulator for both ATMTel1 and ATRMec1. Recently, MRNMRX has also been found to assist in TopBP1Dpb11 recruitment to ssDNA-dsDNA junctions, explaining how this complex might help activate ATRMec1 in Xenopus egg extracts [89,322]. It is proposed that MRNMRX recruits TopBP1Dpb11, after which the interaction between 9-1-1 and TopBP1Dpb11 subsequently exposes the ATRMec1 activation domain (AAD) of TopBP1Dpb11 to fully activate ATRMec1 . On the other hand, the Mre11 nuclease activity has also been implicated in checkpoint activation, possibly by generating ssDNA . In budding yeast MRX is also recruited to stalled replication forks, where it contributes to the maintenance of stalled polymerases . Another study by the same group has demonstrated that MRX has an important role in cohesin association behind the fork in response to HU, which is necessary for fork restart [323,324]. Whereas the full molecular details of how MRX contributes to fork stability and cohesin recruitment remain to be clarified, current results suggest that it may be its structural properties, rather than its nuclease activity, that are crucial [323,324]. In mammalian cells cohesin is also important for CHK2Rad53 activation in the intra-S and the G2/M damage checkpoints, and this role is independent of cohesin’s role in sister chromatid cohesion . In S. pombe, the N-terminus of the MRN component Nbs1Xrs2 is required for the Rad3Mec1-Rad26Ddc2-dependent telomere recruitment of Tel1, while the well-studied Tel1-binding C-terminal Nbs1Xrs2 domain is not needed . These observations further emphasize the significant cooperation that exists between ATRMec1 and ATMTel1, and the important role MRNMRX plays in coordinating their interplay.
The replication checkpoint is a highly conserved surveillance mechanism. Here we have summarized both past and recent insights into the mechanistic aspects of the DNA damage checkpoint response. We focused on the replication checkpoint because of the important role played by the DNA damage response to DNA replication stress in the early stages of human tumorigenesis. It has been amply demonstrated that the DNA damage response, monitored as γH2AX or 53BP1Rad9 foci formation, or CHK2Rad53 phosphorylation is constitutively activated in precancerous lesions, even in the absence of exogenous damage [327,328]. It has been suggested that the DNA damage checkpoint is an important barrier for suppressing cancer development, acting at least in part through p53-dependent senescence [7,327,328,329,330]. The persistent DNA damage response that is detected in precancerous lesions or in hyperplastic tissues results from inappropriate initiation of DNA replication and the ensuing DNA replication stress [329,330]. We note that p53 activation in response to DSBs is primarily mediated by ATMTel1 and CHK2Rad53 , whereas the apoptosis induced by replication stress is independent of p53 [332,333,334]. Thus, p53-deficient cancer cells may rely on ATRMec1-CHK1Chk1 for their survival during replication stress or fork-associated DNA damage. This type of damage is often enhanced by drugs used as chemotherapeutics, namely alkylating chemicals, cis-platin, aphdicolin, or HU. Indeed, ATRMec1 inhibition has been shown to exacerbate the toxicity of replication stress in p53-deficient cells , and CHK1Chk1 inhibition sensitizes p53 mutants to ionizing radiation . Thus, the ATRMec1-CHK1Chk1 replication checkpoint pathway has great potential for improving cancer chemotherapy strategies. In conclusion, continued research into the replication checkpoint is needed to be able to design novel and more effective chemotherapeutics and combined therapies for human cancer. A deeper understanding of how the replication checkpoint maintains replication fork integrity may enable us to better exploit this natural “Achilles heel” of cancer cells for more effective therapies.
We apologize to all colleagues whose work has not been cited due to space limitations. Support was gratefully received from the Novartis Research Foundation and the FP7 Marie Curie Network, Imaging the DNA Damage Response (Image DDR).
Conflicts of Interest
The authors declare no conflict of interest.
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