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Cells 2017, 6(2), 14; doi:10.3390/cells6020014

Methods for Measuring Autophagy in Mice

INSERM, UMR-1048, Institute of Metabolic and Cardiovascular Diseases and University Paul Sabatier, F-31342 Toulouse, France
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Author to whom correspondence should be addressed.
Academic Editor: Fulvio Reggiori
Received: 2 May 2017 / Revised: 2 June 2017 / Accepted: 3 June 2017 / Published: 8 June 2017
(This article belongs to the Special Issue Assays to Monitor Autophagy in Model Systems)
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Abstract

Autophagy is a dynamic intracellular process that mediates the degradation of damaged cytoplasmic components by the lysosome. This process plays important roles in maintaining normal cellular homeostasis and energy balance. Measuring autophagy activity is critical and although the determination of autophagic flux in isolated cells is well documented, there is a need to have reliable and quantitative assays to evaluate autophagy in whole organisms. Because mouse models have been precious in establishing the functional significance of autophagy under physiological or pathological conditions, we present in this chapter a compendium of the current available methods to measure autophagy in mice, and discuss their advantages and limitations. View Full-Text
Keywords: autophagy; methods; flux; mouse models autophagy; methods; flux; mouse models
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Moulis, M.; Vindis, C. Methods for Measuring Autophagy in Mice. Cells 2017, 6, 14.

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