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Erratum published on 20 September 2016, see Cells 2016, 5(3), 36.

Open AccessArticle
Cells 2016, 5(2), 19; doi:10.3390/cells5020019

Generation and Characterization of Vascular Smooth Muscle Cell Lines Derived from a Patient with a Bicuspid Aortic Valve

1
Department of Cardiac and Thoracic Vascular Surgery, University of Luebeck, D-23538 Luebeck, Germany
2
Center of Human Genetics and Genetic Counselling, University of Bremen, D-28359 Bremen, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander E. Kalyuzhny
Received: 6 September 2015 / Revised: 1 March 2016 / Accepted: 11 April 2016 / Published: 21 April 2016
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Abstract

Thoracic aortic dilation is the most common malformation of the proximal aorta and is responsible for 1%–2% of all deaths in industrialized countries. In approximately 50% of patients with a bicuspid aortic valve (BAV), dilation of any or all segments of the aorta occurs. BAV patients with aortic dilation show an increased incidence of cultured vascular smooth muscle cell (VSMC) loss. In this study, VSMC, isolated from the ascending aorta of BAV, was treated with Simian virus 40 to generate a BAV-originated VSMC cell line. To exclude any genomic DNA or cross-contamination, highly polymorphic short tandem repeats of the cells were profiled. The cells were then characterized using flow cytometry and karyotyping. The WG-59 cell line created is the first reported VSMC cell line isolated from a BAV patient. Using an RT2 Profiler PCR Array, genes within the TGFβ/BMP family that are dependent on losartan treatment were identified. Endoglin was found to be among the regulated genes and was downregulated in WG-59 cells following treatment with different losartan concentrations, when compared to untreated WG-59 cells. View Full-Text
Keywords: bicuspid aortic valve; vascular smooth muscle cell; WG-59 cell line; losartan bicuspid aortic valve; vascular smooth muscle cell; WG-59 cell line; losartan
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lazar-Karsten, P.; Belge, G.; Schult-Badusche, D.; Focken, T.; Radtke, A.; Yan, J.; Ranabhat, P.; Mohamed, S.A. Generation and Characterization of Vascular Smooth Muscle Cell Lines Derived from a Patient with a Bicuspid Aortic Valve. Cells 2016, 5, 19.

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