Cells 2012, 1(4), 1029-1044; doi:10.3390/cells1041029
Article

A Novel Type III Endosome Transmembrane Protein, TEMP

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Received: 15 October 2012; in revised form: 26 October 2012 / Accepted: 30 October 2012 / Published: 5 November 2012
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: As part of a high-throughput subcellular localisation project, the protein encoded by the RIKEN mouse cDNA 2610528J11 was expressed and identified to be associated with both endosomes and the plasma membrane. Based on this, we have assigned the name TEMP for Type III Endosome Membrane Protein. TEMP encodes a short protein of 111 amino acids with a single, alpha-helical transmembrane domain. Experimental analysis of its membrane topology demonstrated it is a Type III membrane protein with the amino-terminus in the lumenal, or extracellular region, and the carboxy-terminus in the cytoplasm. In addition to the plasma membrane TEMP was localized to Rab5 positive early endosomes, Rab5/Rab11 positive recycling endosomes but not Rab7 positive late endosomes. Video microscopy in living cells confirmed TEMP's plasma membrane localization and identified the intracellular endosome compartments to be tubulovesicular. Overexpression of TEMP resulted in the early/recycling endosomes clustering at the cell periphery that was dependent on the presence of intact microtubules. The cellular function of TEMP cannot be inferred based on bioinformatics comparison, but its cellular distribution between early/recycling endosomes and the plasma membrane suggests a role in membrane transport.
Keywords: endosome; subcellular localisation; membrane protein
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MDPI and ACS Style

Aturaliya, R.N.; Kerr, M.C.; Teasdale, R.D. A Novel Type III Endosome Transmembrane Protein, TEMP. Cells 2012, 1, 1029-1044.

AMA Style

Aturaliya RN, Kerr MC, Teasdale RD. A Novel Type III Endosome Transmembrane Protein, TEMP. Cells. 2012; 1(4):1029-1044.

Chicago/Turabian Style

Aturaliya, Rajith N.; Kerr, Markus C.; Teasdale, Rohan D. 2012. "A Novel Type III Endosome Transmembrane Protein, TEMP." Cells 1, no. 4: 1029-1044.

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