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Agronomy 2018, 8(9), 163; https://doi.org/10.3390/agronomy8090163

Methylome and Epialleles in Rice Epilines Selected for Energy Use Efficiency

1
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Ghent, Belgium
2
Center for Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium
3
BASF Agricultural Solutions Belgium NV, Technologiepark 38, 9052 Ghent, Belgium
4
Bayer CropScience (BioScience), Multi-Crop Breeding Station (MCBS), Chandippa, Shankarpally, Ranga Reddy District-501203, Telangana State, India
*
Author to whom correspondence should be addressed.
Received: 13 July 2018 / Revised: 6 August 2018 / Accepted: 21 August 2018 / Published: 24 August 2018
(This article belongs to the Special Issue The Regulatory Functions of Epigenetic Mechanisms in Plants)
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Abstract

Epigenetics offers important opportunities in breeding to improve the potential yield in a wide variety of crops. Starting from a pure breeder seed lot of a rice (Oryza sativa ssp. indica) inbred population, repeated testing for improved cellular respiration rates and energy use efficiency (EUE) over three generations identified performant epilines with distinct epigenetic signatures and with improved seed yield in field trials. Epiline DNA methylomes were characterized by genome-wide bisulfite sequencing to discern cytosine methylation changes in relation to transcriptome and phenotype. Regional methylation changes were dispersed over the epiline genomes. A number of upstream-associated differentially methylated regions (DMRs) correlated with differentially expressed genes (DEGs) with a role in particular molecular functions like transmembrane transport and protein kinase activity. Targeted bisulfite sequencing confirmed epiline DMRs that anti-correlated with DEGs, identifying putative epialleles that were susceptible for cytosine methylation changes that might affect gene expression and contribute to the phenotype. Chromatin immunoprecipitation sequencing revealed the extensive enrichment of gene-associated histone H3 lysine-4 trimethylation (H3K4me3), which correlated with gene activation and reduced cytosine methylation. Our data indicate that seed formation is prone to epigenetic changes that might be used as a resource in crop improvement. View Full-Text
Keywords: transcriptome; cytosine methylation; post-translational histone H3 modification; cellular respiration; growth; seed yield transcriptome; cytosine methylation; post-translational histone H3 modification; cellular respiration; growth; seed yield
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Schmidt, M.; Byzova, M.; Martens, C.; Peeters, M.; Raj, Y.; Shukla, S.; Verwulgen, T.; De Block, M.; Van Lijsebettens, M. Methylome and Epialleles in Rice Epilines Selected for Energy Use Efficiency. Agronomy 2018, 8, 163.

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