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Polymers 2017, 9(6), 228; doi:10.3390/polym9060228

A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

1,2,†
,
1,3,†
,
4
,
2,* , 3,* and 1,*
1
Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China
2
Nano Science and Technology Institute, University of Science and Technology of China, 96 Jin Zhai Road, Hefei 230026, China
3
Department of Chemical and Molecular Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing 211816, China
4
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China
Yanqiu Liu and Yaqi Ding contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Böker
Received: 17 May 2017 / Revised: 7 June 2017 / Accepted: 10 June 2017 / Published: 16 June 2017
View Full-Text   |   Download PDF [8188 KB, uploaded 16 June 2017]   |  

Abstract

A heterogalactan, named ACW0, was extracted from Antrodia camphorata and purified by anion exchange and gel permeation chromatography. It was composed of galactose (94.98%), traces of mannose (2.41%), and fucose (2.61%), with its molecular weight estimated to be 13.5 k Da. The polysaccharide ACW0 was shown to be a mannofucogalactan with a backbone chain of α-d-1,6-linked Gal, attached by a non-reducing terminal α-d-Man and α-l-Fuc on C-2 of nearly every six α-d-1,6-linked Gal residues. A sulfated polysaccharide, ACW0-Sul was achieved by the chlorosulfonic acid-pyridine method. Compared with the native polysaccharide, ACW0-Sul could disrupt tube formation and migration as well as cell growth of human microvascular endothelial cells (HMEC-1) dose-dependently. Further studies revealed that phosphorylation of Extracellular Regulated Protein Kinases (Erk) and Focal Adhesion Kinase (FAK) were significantly inhibited by ACW0-Sul. These results suggested that ACW0-Sul could be a potent candidate for anti-angiogenic agent development. View Full-Text
Keywords: Antrodia camphorata; mannofucogalactan; sulfated polysaccharide; anti-angiogenesis Antrodia camphorata; mannofucogalactan; sulfated polysaccharide; anti-angiogenesis
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Liu, Y.; Ding, Y.; Ye, M.; Zhu, T.; Tian, D.; Ding, K. A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative. Polymers 2017, 9, 228.

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