Next Article in Journal
DNA Compaction and Charge Inversion Induced by Organic Monovalent Ions
Next Article in Special Issue
A Facile Approach for Fabrication of Core-Shell Magnetic Molecularly Imprinted Nanospheres towards Hypericin
Previous Article in Journal / Special Issue
A Gelated Colloidal Crystal Attached Lens for Noninvasive Continuous Monitoring of Tear Glucose
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessCommunication
Polymers 2017, 9(4), 127; doi:10.3390/polym9040127

Label-Free Colorimetric Detection of Influenza Antigen Based on an Antibody-Polydiacetylene Conjugate and Its Coated Polyvinylidene Difluoride Membrane

1
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Center for Biotechnology Research in UBITA (CBRU), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
2
Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Applications (UBITA), Konkuk University, 120 Neungdong-ro, Gwangjingu, Seoul 05029, Korea
3
Department of Biological Sciences, Konkuk University, 120 Neungdong-ro, Gwangjingu, Seoul 05029, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Po-Chih Yang
Received: 27 February 2017 / Revised: 25 March 2017 / Accepted: 27 March 2017 / Published: 30 March 2017
(This article belongs to the Special Issue Polymers for Chemosensing)
View Full-Text   |   Download PDF [2862 KB, uploaded 30 March 2017]   |  

Abstract

This study presents an antibody-conjugated polydiacetylene (PDA) and its coated polyvinylidene difluoride (PVDF) membrane. The M149 antibody was hybridized to nano-vesicles consisting of pentacosa-10,12-diynoic acid (PCDA) and dimyristoylphosphatidylcholine (DMPC). After photo-polymerization at 254 nm, the effects on the PDA by antigenic injection were investigated with UV-vis spectroscopy, fluorescence spectroscopy, dynamic light scattering and transmission electron microscopy. Because PDA, an alternating ene-yne molecule, induces a blue-to-red color transition and an interesting fluorescent response by the distortion of its backbone, the biomolecular recognition of an antibody–antigen can be converted into an optical and fluorescent signal. Thus, an influenza antigen was successfully detected with the proposed label-free method. Furthermore, the vesicular system was improved by coating it onto a membrane type sensing platform for its stability and portability. The proposed antibody-PDA composite PVDF membrane has potential for rapid, easy and selective visualization of the influenza virus. View Full-Text
Keywords: antibody; polydiacetylene; polyvinylidene difluoride; label-free detection; influenza virus antibody; polydiacetylene; polyvinylidene difluoride; label-free detection; influenza virus
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jeong, J.-P.; Cho, E.; Yun, D.; Kim, T.; Lee, I.-S.; Jung, S. Label-Free Colorimetric Detection of Influenza Antigen Based on an Antibody-Polydiacetylene Conjugate and Its Coated Polyvinylidene Difluoride Membrane. Polymers 2017, 9, 127.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top