Next Article in Journal
The Impact of Polyvinylpyrrolidone on Properties of Cadmium Oxide Semiconductor Nanoparticles Manufactured by Heat Treatment Technique
Next Article in Special Issue
Effect of Small Reaction Locus in Free-Radical Polymerization: Conventional and Reversible-Deactivation Radical Polymerization
Previous Article in Journal
Fretting Fatigue Behaviour of Pin-Loaded Thermoset Carbon-Fibre-Reinforced Polymer (CFRP) Straps
Previous Article in Special Issue
Green Synthesis of Smart Metal/Polymer Nanocomposite Particles and Their Tuneable Catalytic Activities
Article Menu

Export Article

Open AccessArticle
Polymers 2016, 8(4), 116; doi:10.3390/polym8040116

Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres

1
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Academic Editors: Katja Loos and Frank Wiesbrock
Received: 26 January 2016 / Revised: 6 March 2016 / Accepted: 24 March 2016 / Published: 7 April 2016
(This article belongs to the Special Issue Selected Papers from ASEPFPM2015)
View Full-Text   |   Download PDF [5445 KB, uploaded 7 April 2016]   |  

Abstract

Compared with the one immobilized in the conventional mesoporous microspheres, the enzyme immobilized in gigaporous microspheres showed much higher activity and better stability. To gain a deeper understanding, we herein selected lipase as a prototype to comparatively analyze the adsorption behavior of lipase at interfaces in gigaporous and mesoporous polystyrene microspheres at very low lipase concentration, and further compared with the adsorption on a completely flat surface (a chip). Owing to the limited space of narrow pores, lipase molecules were inclined to be adsorbed as a monolayer in mesoporous microspheres. During this process, the interaction between lipase molecules and the interface was stronger, which could result in the structural change of lipase molecular and compromised specific activity. In addition to monolayer adsorption, more multilayer adsorption of enzyme molecules also occurred in gigaporous microspheres. Besides the adsorption state, the pore curvature also affected the lipase adsorption. Due to the multilayer adsorption, the excellent mass transfer properties for the substrate and the product in the large pores, and the small pore curvature, lipase immobilized in gigaporous microspheres showed better behaviors. View Full-Text
Keywords: lipase; adsorption; gigaporous; mesoporous; microsphere; QCM-D lipase; adsorption; gigaporous; mesoporous; microsphere; QCM-D
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, W.; Zhou, W.; Wei, W.; Li, J.; Hao, D.; Su, Z.; Ma, G. Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres. Polymers 2016, 8, 116.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top