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Polymers 2016, 8(3), 74; doi:10.3390/polym8030074

Fabrication of Alkoxyamine-Functionalized Magnetic Core-Shell Microspheres via Reflux Precipitation Polymerization for Glycopeptide Enrichment

1
Department of Macromolecular Science, State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials, Fudan University, Shanghai 200433, China
2
Institutes of Biomedical Sciences and Department of Chemistry, Fudan University, Shanghai 200032, China
These authors contributed equally.
*
Authors to whom correspondence should be addressed.
Academic Editor: Guanghui Ma
Received: 19 January 2016 / Revised: 14 February 2016 / Accepted: 19 February 2016 / Published: 4 March 2016
(This article belongs to the Special Issue Selected Papers from ASEPFPM2015)
View Full-Text   |   Download PDF [4387 KB, uploaded 4 March 2016]   |  

Abstract

As a facile method to prepare hydrophilic polymeric microspheres, reflux precipitation polymerization has been widely used for preparation of polymer nanogels. In this article, we synthesized a phthalamide-protected N-aminooxy methyl acrylamide (NAMAm-p) for preparation of alkoxyamine-functionalized polymer composite microspheres via reflux precipitation polymerization. The particle size and functional group density of the composite microspheres could be adjusted by copolymerization with the second monomers, N-isopropyl acrylamide, acrylic acid or 2-hydroxyethyl methacrylate. The resultant microspheres have been characterized by TEM, FT-IR, TGA and DLS. The experimental results showed that the alkoxyamine group density of the microspheres could reach as high as 1.49 mmol/g, and these groups showed a great reactivity with ketone/aldehyde compounds. With the aid of magnetic core, the hybrid microspheres could capture and magnetically isolate glycopeptides from the digested mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio. After that, we applied the composite microspheres to profile the glycol-proteome of a normal human serum sample, 95 unique glycopeptides and 64 glycoproteins were identified with these enrichment substrates in a 5 μL of serum sample. View Full-Text
Keywords: alkoxyamine-functionalized microspheres; reflux precipitation polymerization; magnetic composite microspheres; oxime click; glycoproteins/glycopeptides enrichment alkoxyamine-functionalized microspheres; reflux precipitation polymerization; magnetic composite microspheres; oxime click; glycoproteins/glycopeptides enrichment
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yu, M.; Di, Y.; Zhang, Y.; Zhang, Y.; Guo, J.; Lu, H.; Wang, C. Fabrication of Alkoxyamine-Functionalized Magnetic Core-Shell Microspheres via Reflux Precipitation Polymerization for Glycopeptide Enrichment. Polymers 2016, 8, 74.

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