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Polymers 2015, 7(8), 1427-1443; doi:10.3390/polym7081427

Stable Poly(methacrylic acid) Brush Decorated Silica Nano-Particles by ARGET ATRP for Bioconjugation

1
School of Chemical Sciences, Dublin City University, Dublin 9, Ireland
2
Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland
3
School of Pharmacy, Royal College of Surgeons in Ireland, Dublin 2, Ireland
*
Author to whom correspondence should be addressed.
Academic Editor: Walter Remo Caseri
Received: 15 June 2015 / Revised: 27 July 2015 / Accepted: 28 July 2015 / Published: 6 August 2015
View Full-Text   |   Download PDF [3022 KB, uploaded 6 August 2015]   |  

Abstract

The synthesis of polymer brush decorated silica nano-particles is demonstrated by activator regeneration by electron transfer atom transfer radical polymerization (ARGET ATRP) grafting of poly(tert-butyl methacrylate). ATRP initiator decorated silica nano-particles were obtained using a novel trimethylsiloxane derivatised ATRP initiator obtained by click chemistry. Comparison of de-grafted polymers with polymer obtained from a sacrificial initiator demonstrated good agreement up to 55% monomer conversion. Subsequent mild deprotection of the tert-butyl ester groups using phosphoric acid yielded highly colloidal and pH stable hydrophilic nano-particles comprising approximately 50% methacrylic acid groups. The successful bio-conjugation was achieved by immobilization of Horseradish Peroxidase to the polymer brush decorated nano-particles and the enzyme activity demonstrated in a conversion of o-phenylene diamine dihydrochloride assay. View Full-Text
Keywords: silica nano-particles; polymer brushes; ARGET ATRP; poly(methacrylic acid); bio-conjugation; enzyme immobilization silica nano-particles; polymer brushes; ARGET ATRP; poly(methacrylic acid); bio-conjugation; enzyme immobilization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Iacono, M.; Heise, A. Stable Poly(methacrylic acid) Brush Decorated Silica Nano-Particles by ARGET ATRP for Bioconjugation. Polymers 2015, 7, 1427-1443.

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