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Polymers 2015, 7(10), 2131-2145; doi:10.3390/polym7101503

Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection

Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Chia-Yi 621, Taiwan
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Author to whom correspondence should be addressed.
Academic Editor: Jianxun Ding
Received: 2 August 2015 / Revised: 13 October 2015 / Accepted: 15 October 2015 / Published: 23 October 2015
(This article belongs to the Special Issue Functional Polymers for Medical Applications)
View Full-Text   |   Download PDF [3073 KB, uploaded 23 October 2015]   |  

Abstract

A polyurethane (PU) grafted with small molecular weight polyethylenimine (PEI600) was synthesized. This PU-PEI600 can assemble DNA via electrostatic interactions into nano-sized polymer/DNA complexes. The complexes exhibited great transfection efficiency in delivering DNA along with a reduced cell toxicity comparing to commercial PEI25k (Mw ~25,000). In order to establish a system for concurrently delivering two different DNA or RNA molecules for cell reprogramming (e.g., induced pluripotent stem cells) or the necessity of multi-expression (e.g., double knock down), the PU-PEI600 was further functionalized with maleimide molecules. The novel PU-PEI600-maleimide would still effectively interact with assigned DNA and different functions of PU-PEI600-maleimide/DNA complexes were self-conjugated in presence of a dithiol molecule (1,6-hexanedithiol). In this study, two reporter genes (pEGFP-C2 and pLanRFP-N) were used and evidence of green/red fluorescence co-expression in cells was observed. This article brings a new concept and a practical method for a plurality of different DNA molecules that are more efficient to be concurrently delivered and co-expressed. This method is very helpful in studying cellular multi-regulation or in the treatment of disease with multiple gene defects in vivo. View Full-Text
Keywords: DNA transfection; polyurethane; PEI; maleimide; thiol conjugation DNA transfection; polyurethane; PEI; maleimide; thiol conjugation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hung, W.-C.; Cherng, J.-Y. Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection. Polymers 2015, 7, 2131-2145.

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