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Polymers 2014, 6(5), 1631-1654; doi:10.3390/polym6051631
Article

Development of a Biocompatible Layer-by-Layer Film System Using Aptamer Technology for Smart Material Applications

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Received: 17 March 2014; in revised form: 4 May 2014 / Accepted: 8 May 2014 / Published: 23 May 2014
(This article belongs to the Special Issue Polyelectrolytes 2014)
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Abstract: Aptamers are short, single-stranded nucleic acids that fold into well-defined three dimensional (3D) structures that allow for binding to a target molecule with affinities and specificities that can rival or in some cases exceed those of antibodies. The compatibility of aptamers with nanostructures such as thin films, in combination with their affinity, selectivity, and conformational changes upon target interaction, could set the foundation for the development of novel smart materials. In this study, the development of a biocompatible aptamer-polyelectrolyte film system was investigated using a layer-by-layer approach. Using fluorescence microscopy, we demonstrated the ability of the sulforhodamine B aptamer to bind its cognate target while sequestered in a chitosan-hyaluronan film matrix. Studies using Ultraviolet-visible (UV-Vis) spectrophotometry also suggest that deposition conditions such as rinsing time and volume play a strong role in the internal film interactions and growth mechanisms of chitosan-hyaluronan films. The continued study and development of aptamer-functionalized thin films provides endless new opportunities for novel smart materials and has the potential to revolutionize the field of controlled release.
Keywords: aptamer; sulforhodamine B; layer-by-layer; chitosan; hyaluronan; exponential growth; linear growth; polyelectrolyte aptamer; sulforhodamine B; layer-by-layer; chitosan; hyaluronan; exponential growth; linear growth; polyelectrolyte
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Foster, A.; DeRosa, M.C. Development of a Biocompatible Layer-by-Layer Film System Using Aptamer Technology for Smart Material Applications. Polymers 2014, 6, 1631-1654.

AMA Style

Foster A, DeRosa MC. Development of a Biocompatible Layer-by-Layer Film System Using Aptamer Technology for Smart Material Applications. Polymers. 2014; 6(5):1631-1654.

Chicago/Turabian Style

Foster, Amanda; DeRosa, Maria C. 2014. "Development of a Biocompatible Layer-by-Layer Film System Using Aptamer Technology for Smart Material Applications." Polymers 6, no. 5: 1631-1654.


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