Polymers 2012, 4(1), 674-690; doi:10.3390/polym4010674
Article

Synthesis of Hyperbranched Glycoconjugates by the Combined Action of Potato Phosphorylase and Glycogen Branching Enzyme from Deinococcus geothermalis

Received: 2 January 2012; in revised form: 31 January 2012 / Accepted: 8 February 2012 / Published: 27 February 2012
(This article belongs to the Special Issue Enzymes in Monomer and Polymer Synthesis)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Potato phosphorylase is able to synthesize linear polyglucans from maltoheptaose primers. By coupling maltoheptaose to butane diamine, tris(2-aminoethyl)amine and amine functionalized amine functionalized poly ethyleneglycol (PEG), new primer molecules became available. The resulting di-, tri- and macro-primers were incubated with potato phosphorylase and glycogen branching enzyme from Deinococcus geothermalis. Due to the action of both enzymes, hyperbranched polyglucan arms were grown from the maltoheptaose derivatives with a maximum degree of branching of 11%. The size of the synthesized hyperbranched polyglucans could be controlled by the ratio monomer over primer. About 60%–80% of the monomers were incorporated in the glycoconjugates. The resulting hyperbranched glycoconjugates were subjected to Dynamic Light Scattering (DLS) measurements in order to determine the hydrodynamic radius and it became obvious that the structures formed agglomerates in the range of 14–32 nm.
Keywords: glycoconjugates; biohybrids; glycosyltransferases; branching enzyme; potato phosphorylase; hyperbranched; enzymatic polymerization
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MDPI and ACS Style

Vlist, J.; Faber, M.; Loen, L.; Dijkman, T.J.; Asri, L.A.T.W.; Loos, K. Synthesis of Hyperbranched Glycoconjugates by the Combined Action of Potato Phosphorylase and Glycogen Branching Enzyme from Deinococcus geothermalis. Polymers 2012, 4, 674-690.

AMA Style

Vlist J, Faber M, Loen L, Dijkman TJ, Asri LATW, Loos K. Synthesis of Hyperbranched Glycoconjugates by the Combined Action of Potato Phosphorylase and Glycogen Branching Enzyme from Deinococcus geothermalis. Polymers. 2012; 4(1):674-690.

Chicago/Turabian Style

Vlist, Jeroen van der; Faber, Martin; Loen, Lizette; Dijkman, Teunis J.; Asri, Lia A. T. W.; Loos, Katja. 2012. "Synthesis of Hyperbranched Glycoconjugates by the Combined Action of Potato Phosphorylase and Glycogen Branching Enzyme from Deinococcus geothermalis." Polymers 4, no. 1: 674-690.

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