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Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization
Polymer Chemistry & Biomaterials Research Group, Ghent University, Krijgslaan 281 S4-bis, 9000 Ghent, Belgium
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Received: 11 November 2010; in revised form: 10 December 2010 / Accepted: 31 December 2010 / Published: 5 January 2011
Abstract: In the present work, the immobilization of gelatin as biopolymer on two types of implantable biomaterials, polyimide and titanium, was compared. Both materials are known for their biocompatibility while lacking cell-interactive behavior. For both materials, a pre-functionalization step was required to enable gelatin immobilization. For the polyimide foils, a reactive succinimidyl ester was introduced first on the surface, followed by covalent grafting of gelatin. For the titanium material, methacrylate groups were first introduced on the Ti surface through a silanization reaction. The applied functionalities enabled the subsequent immobilization of methacrylamide modified gelatin. Both surface modified materials were characterized in depth using atomic force microscopy, static contact angle measurements, confocal fluorescence microscopy, attenuated total reflection infrared spectroscopy and X-ray photo-electron spectroscopy. The results indicated that the strategies elaborated for both material classes are suitable to apply stable gelatin coatings. Interestingly, depending on the material class studied, not all surface analysis techniques are applicable.
Keywords: gelatin immobilization; polyimide; titanium; surface characterization
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Van Vlierberghe, S.; Vanderleyden, E.; Boterberg, V.; Dubruel, P. Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization. Polymers 2011, 3, 114-130.
Van Vlierberghe S, Vanderleyden E, Boterberg V, Dubruel P. Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization. Polymers. 2011; 3(1):114-130.
Van Vlierberghe, Sandra; Vanderleyden, Els; Boterberg, Veerle; Dubruel, Peter. 2011. "Gelatin Functionalization of Biomaterial Surfaces: Strategies for Immobilization and Visualization." Polymers 3, no. 1: 114-130.