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Polymers 2018, 10(8), 839; https://doi.org/10.3390/polym10080839

The Effect of Hexanoyl Glycol Chitosan on the Proliferation of Human Mesenchymal Stem Cells

1
Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Korea
2
Department of Polymer Science and Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
3
Soonchunhyang Institute of Medi-bio Science (SIMS), Soon Chun Hyang University, Cheonan 31151, Korea
4
Predictive Model Research Center, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Korea
5
Department of Human and Environmental Toxicology, University of Science and Technology, Daejeon 34114, Korea
6
Department of Medicine, School of Medicine, Konkuk University, Seoul 05029, Korea
These authors contributed equally to this work as first author.
These authors contributed equally to this work as corresponding authors.
*
Authors to whom correspondence should be addressed.
Received: 2 July 2018 / Revised: 25 July 2018 / Accepted: 27 July 2018 / Published: 30 July 2018
(This article belongs to the Special Issue Polymeric Micro/Nanoparticles for Bio-Medical Applications)
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Abstract

Adipose-derived mesenchymal stem cells (AD-MSCs) have been studied as desirable cell sources for regenerative medicine and therapeutic application. However, it has still remained a challenge to obtain enough adequate and healthy cells in large quantities. To overcome this limitation, various biomaterials have been used to promote expansion of MSCs in vitro. Recently, hexanoyl glycol chitosan (HGC) was introduced as a new biomaterial for various biomedical applications, in particular 3D cell culture, because of its biodegradability, biocompatibility, and other promising biofunctional properties. In this study, the effect of HGC on the proliferation of AD-MSCs was examined in vitro, and its synergistic effect with basic fibroblast growth factor (bFGF), which has been widely used to promote proliferation of cells, was evaluated. We found that the presence of HGC increased the proliferative capacity of AD-MSCs during long-term culture, even at low concentrations of bFGF. Furthermore, it suppressed the expression of senescence-related genes and improved the mitochondrial functionality. Taken all together, these findings suggest that the HGC demonstrate a potential for sustained growth of AD-MSCs in vitro. View Full-Text
Keywords: mesenchymal stem cell; hexanoyl glycol chitosan; basic fibroblast growth factor mesenchymal stem cell; hexanoyl glycol chitosan; basic fibroblast growth factor
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Jeong, Y.-H.; Oh, H.M.; Lee, M.R.; Kim, C.-Y.; Joo, C.; Park, S.-J.; Song, Y.-H.; Kang, C.; Chung, H.-M.; Kang, S.-W.; Huh, K.M.; Moon, S.-H. The Effect of Hexanoyl Glycol Chitosan on the Proliferation of Human Mesenchymal Stem Cells. Polymers 2018, 10, 839.

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