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Polymers 2018, 10(6), 589; https://doi.org/10.3390/polym10060589

Size-Controllable Enzymatic Synthesis of Short Hairpin RNA Nanoparticles by Controlling the Rate of RNA Polymerization

Department of Chemical Engineering, University of Seoul, Seoul 02504, Korea
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Received: 12 April 2018 / Revised: 22 May 2018 / Accepted: 23 May 2018 / Published: 28 May 2018
(This article belongs to the Special Issue Polymerization Kinetics)
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Abstract

Thanks to a wide range of biological functions of RNA, and advancements in nanotechnology, RNA nanotechnology has developed in multiple ways for RNA-based therapeutics. In particular, among RNA engineering techniques, enzymatic self-assembly of RNA structures has gained great attention for its high packing density of RNA, with a low cost and one-pot synthetic process. However, manipulation of the overall size of particles, especially a reduction in size, has not been studied in depth. Here, we reported the enzymatic self-assembly of short hairpin RNA particles for the downregulation of target genes, and a rational approach to the manipulation of the resultant particle size. This is the first report of the size-controllable enzymatic self-assembly of short hairpin RNA nanoparticles. While keeping all the benefits of an enzymatic approach, the overall size of the RNA particles was controlled on a scale of 2 μm to 100 nm, falling within the therapeutically applicable size range. View Full-Text
Keywords: RNA nanotechnology; RNA therapeutics; short hairpin RNA nanoparticles; enzymatic self-assembly RNA nanotechnology; RNA therapeutics; short hairpin RNA nanoparticles; enzymatic self-assembly
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Kim, H.; Kim, D.; Jeong, J.; Jeon, H.; Lee, J.B. Size-Controllable Enzymatic Synthesis of Short Hairpin RNA Nanoparticles by Controlling the Rate of RNA Polymerization. Polymers 2018, 10, 589.

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