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Polymers 2018, 10(4), 463; https://doi.org/10.3390/polym10040463

Multienzyme Immobilized Polymeric Membrane Reactor for the Transformation of a Lignin Model Compound

1
Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506, USA
2
Department of Biology, University of Louisville, Louisville, KY 40292, USA
*
Author to whom correspondence should be addressed.
Received: 29 March 2018 / Revised: 17 April 2018 / Accepted: 20 April 2018 / Published: 23 April 2018
(This article belongs to the Special Issue Surface Modification and Functional Coatings for Polymers)
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Abstract

We have developed an integrated, multienzyme functionalized membrane reactor for bioconversion of a lignin model compound involving enzymatic catalysis. The membrane bioreactors were fabricated through the layer-by-layer assembly approach to immobilize three different enzymes (glucose oxidase, peroxidase and laccase) into pH-responsive membranes. This novel membrane reactor couples the in situ generation of hydrogen peroxide (by glucose oxidase) to oxidative conversion of a lignin model compound, guaiacylglycerol-β-guaiacyl ether (GGE). Preliminary investigation of the efficacy of these functional membranes towards GGE degradation is demonstrated under convective flow mode. Over 90% of the initial feed could be degraded with the multienzyme immobilized membranes at a residence time of approximately 22 s. GGE conversion product analysis revealed the formation of oligomeric oxidation products upon reaction with peroxidase, which may be a potential hazard to membrane bioreactors. These oxidation products could further be degraded by laccase enzymes in the multienzymatic membranes, explaining the potential of multi enzyme membrane reactors. The multienzyme incorporated membrane reactors were active for more than 30 days of storage time at 4 °C. During this time span, repetitive use of the membrane reactor was demonstrated involving 5–6 h of operation time for each cycle. The membrane reactor displayed encouraging performance, losing only 12% of its initial activity after multiple cycles of operation. View Full-Text
Keywords: polymer membrane reactor; multienzyme; lignin; layer-by-layer assembly polymer membrane reactor; multienzyme; lignin; layer-by-layer assembly
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Sarma, R.; Islam, M.S.; Running, M.P.; Bhattacharyya, D. Multienzyme Immobilized Polymeric Membrane Reactor for the Transformation of a Lignin Model Compound. Polymers 2018, 10, 463.

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