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Polymers 2018, 10(4), 452; https://doi.org/10.3390/polym10040452

Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

1
Center for Natural and Human Sciences, Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP, CEP 09210-580, Brazil
2
Nanomedicine Research Unit (NANOMED), Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP 09210-580, Brazil
3
Tropical Disease Laboratory, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP 13083-862, Brazil
4
Center for Engineering, Modeling and Applied Social Science, Universidade Federal do ABC, Alameda da Universidade sem numero, São Bernardo do Campo, SP, CEP 09606-045, Brazil
*
Author to whom correspondence should be addressed.
Received: 16 March 2018 / Revised: 8 April 2018 / Accepted: 16 April 2018 / Published: 18 April 2018
(This article belongs to the Special Issue Polymers for Therapy and Diagnostics)
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Abstract

Nitric oxide (NO) is involved in physiological processes, including vasodilatation, wound healing and antibacterial activities. As NO is a free radical, designing drugs to generate therapeutic amounts of NO in controlled spatial and time manners is still a challenge. In this study, the NO donor S-nitrosoglutathione (GSNO) was incorporated into the thermoresponsive Pluronic F-127 (PL)-chitosan (CS) hydrogel, with an easy and economically feasible methodology. CS is a polysaccharide with known antimicrobial properties. Scanning electron microscopy, rheology and differential scanning calorimetry techniques were used for hydrogel characterization. The results demonstrated that the hydrogel has a smooth surface, thermoresponsive behavior and good mechanical stability. The kinetics of NO release and GSNO diffusion from GSNO-containing PL/CS hydrogel demonstrated a sustained NO/GSNO release, in concentrations suitable for biomedical applications. The GSNO-PL/CS hydrogel demonstrated a concentration-dependent toxicity to Vero cells, and antimicrobial activity to Pseudomonas aeruginosa (minimum inhibitory concentration and minimum bactericidal concentration values of 0.5 µg·mL−1 of hydrogel, which corresponds to 1 mmol·L−1 of GSNO). Interestingly, the concentration range in which the NO-releasing hydrogel demonstrated an antibacterial effect was not found to be toxic to the Vero mammalian cell. Thus, the GSNO-PL/CS hydrogel is a suitable biomaterial for topical NO delivery applications. View Full-Text
Keywords: chitosan; thermoresponsive hydrogel; nitric oxide; S-nitrosothiols; biocompatibility; antimicrobial; Pseudomonas aeruginosa; Pluronic F127 chitosan; thermoresponsive hydrogel; nitric oxide; S-nitrosothiols; biocompatibility; antimicrobial; Pseudomonas aeruginosa; Pluronic F127
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pelegrino, M.T.; de Araujo Lima, B.; do Nascimento, M.H.M.; Lombello, C.B.; Brocchi, M.; Seabra, A.B. Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications. Polymers 2018, 10, 452.

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