Site-Specific DBCO Modification of DEC205 Antibody for Polymer Conjugation
AbstractThe design of multifunctional polymer-based vectors, forming pDNA vaccines, offers great potential in cancer immune therapy. The transfection of dendritic immune cells (DCs) with tumour antigen-encoding pDNA leads to an activation of the immune system to combat tumour cells. In this work, we investigated the chemical attachment of DEC205 antibodies (aDEC205) as DC-targeting structures to polyplexes of P(Lys)-b-P(HPMA). The conjugation of a synthetic block copolymer and a biomacromolecule with various functionalities (aDEC205) requires bioorthogonal techniques to avoid side reactions. Click chemistry and in particular the strain-promoted alkyne-azide cycloaddition (SPAAC) can provide the required bioorthogonality. With regard to a SPAAC of both components, we firstly synthesized two different azide-containing block copolymers, P(Lys)-b-P(HPMA)-N3(stat) and P(Lys)-b-P(HPMA)-N3(end), for pDNA complexation. In addition, the site-specific incorporation of ring-strained dibenzocyclooctyne (DBCO) moieties to the DEC205 antibody was achieved by an enzymatic strategy using bacterial transglutaminase (BTG). The chemical accessibility of DBCO molecules within aDEC205 as well as the accessibility of azide-functionalities on the polyplex’ surface were investigated by various SPAAC experiments and characterized by fluorescence correlation spectroscopy (FCS). View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 1142 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Beck, S.; Schultze, J.; Räder, H.-J.; Holm, R.; Schinnerer, M.; Barz, M.; Koynov, K.; Zentel, R. Site-Specific DBCO Modification of DEC205 Antibody for Polymer Conjugation. Polymers 2018, 10, 141.
Beck S, Schultze J, Räder H-J, Holm R, Schinnerer M, Barz M, Koynov K, Zentel R. Site-Specific DBCO Modification of DEC205 Antibody for Polymer Conjugation. Polymers. 2018; 10(2):141.Chicago/Turabian Style
Beck, Simone; Schultze, Jennifer; Räder, Hans-Joachim; Holm, Regina; Schinnerer, Meike; Barz, Matthias; Koynov, Kaloian; Zentel, Rudolf. 2018. "Site-Specific DBCO Modification of DEC205 Antibody for Polymer Conjugation." Polymers 10, no. 2: 141.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.