Next Article in Journal
Polymers – A New Open Access Scientific Journal on Polymer Science
Previous Article in Journal
Mild and Facile Synthesis of Multi-Functional RAFT Chain Transfer Agents
Article Menu

Export Article

Open AccessArticle
Polymers 2009, 1(1), 16-34; doi:10.3390/polym1010016

Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids

1
Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), BP53, 38041 Grenoble cedex 9, France
2
Joseph Fourier University, BP53, 38041 Grenoble cedex 9, France
3
Centre de Rhumatologie, 25, boulevard Agutte Sembat, 38000 Grenoble, France
4
Laboratoire d’exploration et de morphométrie articulaire, Centre hospitalier Lyon –Sud, Pierre-Bénite, France
5
Service de Rhumatologie, Centre hospitalier Lyon –Sud, Pierre-Bénite, France
*
Author to whom correspondence should be addressed.
Received: 28 October 2009 / Revised: 16 December 2009 / Accepted: 28 December 2009 / Published: 28 December 2009
View Full-Text   |   Download PDF [580 KB, uploaded 29 December 2009]   |  

Abstract

Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology.
Keywords: hyaluronan; steric exclusion chromatography; rheology; centrifugation; freezing; filtration; protease treatment hyaluronan; steric exclusion chromatography; rheology; centrifugation; freezing; filtration; protease treatment
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rinaudo, M.; Rozand, Y.; Mathieu, P.; Conrozier, T. Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids. Polymers 2009, 1, 16-34.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top