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Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids
Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), BP53, 38041 Grenoble cedex 9, France
Joseph Fourier University, BP53, 38041 Grenoble cedex 9, France
Centre de Rhumatologie, 25, boulevard Agutte Sembat, 38000 Grenoble, France
Laboratoire d’exploration et de morphométrie articulaire, Centre hospitalier Lyon –Sud, Pierre-Bénite, France
Service de Rhumatologie, Centre hospitalier Lyon –Sud, Pierre-Bénite, France
* Author to whom correspondence should be addressed.
Received: 28 October 2009; in revised form: 16 December 2009 / Accepted: 28 December 2009 / Published: 28 December 2009
Abstract: Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology.
Keywords: hyaluronan; steric exclusion chromatography; rheology; centrifugation; freezing; filtration; protease treatment
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Rinaudo, M.; Rozand, Y.; Mathieu, P.; Conrozier, T. Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids. Polymers 2009, 1, 16-34.
Rinaudo M, Rozand Y, Mathieu P, Conrozier T. Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids. Polymers. 2009; 1(1):16-34.
Rinaudo, Marguerite; Rozand, Yves; Mathieu, Pierre; Conrozier, Thierry. 2009. "Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids." Polymers 1, no. 1: 16-34.